Mammalian Abp1, a Signal-responsive F-Actin-binding Protein, Links the Actin Cytoskeleton to Endocytosis Via the GTPase Dynamin

doi:10.1083/jcb.153.2.351

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Published online 16 April 2001. doi:10.1083/jcb.153.2.351

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© The Rockefeller University Press, 0021-9525/2001/4/351/ $5.00
The Journal of Cell Biology, Volume 153, Number 2, April 16, 2001 351-366

Original Article

Mammalian Abp1, a Signal-responsive F-Actin–binding Protein, Links the Actin Cytoskeleton to Endocytosis Via the GTPase Dynamin

Michael M. Kessels^a, Åsa E.Y. Engqvist-Goldstein^b, David G. Drubin^b, and Britta Qualmann^a
^a Department of Neurochemistry and Molecular Biology, Leibniz Institute for Neurobiology, D-39008 Magdeburg, Germany
^b Department of Molecular and Cell Biology, University of California, Berkeley, California 94720-3202

Correspondence to: Michael M. Kessels, Department of Neurochemistry and Molecular Biology, Leibniz Institute for Neurobiology, Postfach 1860, D-39008 Magdeburg, Germany. Tel:49 391 6263 225 Fax:49 391 6263 229 E-mail:kessels{at}ifn-magdeburg.de.

The actin cytoskeleton has been implicated in endocytosis, yetfew molecular links to the endocytic machinery have been established.Here we show that the mammalian F-actin–binding proteinAbp1 (SH3P7/HIP-55) can functionally link the actin cytoskeletonto dynamin, a GTPase that functions in endocytosis. Abp1 bindsdirectly to dynamin in vitro through its SH3 domain. Coimmunoprecipitationand colocalization studies demonstrated the in vivo relevanceof this interaction. In neurons, mammalian Abp1 and dynamincolocalized at actin-rich sites proximal to the cell body duringsynaptogenesis. In fibroblasts, mAbp1 appeared at dynamin-richsites of endocytosis upon growth factor stimulation. To testwhether Abp1 functions in endocytosis, we overexpressed severalAbp1 constructs in Cos-7 cells and assayed receptor-mediatedendocytosis. While overexpression of Abp1's actin-binding modulesdid not interfere with endocytosis, overexpression of the SH3domain led to a potent block of transferrin uptake. This implicatesthe Abp1/dynamin interaction in endocytic function. The endocytosisblock was rescued by cooverexpression of dynamin. Since theaddition of the actin-binding modules of Abp1 to the SH3 domainconstruct also fully restored endocytosis, Abp1 may supportendocytosis by combining its SH3 domain interactions with cytoskeletalfunctions in response to signaling cascades converging on thislinker protein.

Key Words: actin cytoskeleton, dynamin, endocytosis, SH3P7, neuronal plasticity

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