A Mechanism for Nuclear Positioning in Fission Yeast Based on Microtubule Pushing

doi:10.1083/jcb.153.2.397

A correction to this article has been published: J. Cell Biol. 153 (4) 891-892
Published online 16 April 2001. doi:10.1083/jcb.153.2.397

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© The Rockefeller University Press, 0021-9525/2001//397 $5.00
The Journal of Cell Biology, Volume 153, Number 2, , 2001 397-412

Original Article

A Mechanism for Nuclear Positioning in Fission Yeast Based on Microtubule Pushing

P.T. Tran^a, L. Marsh^b, V. Doye^c, S. Inoué^d, and F. Chang^a

^a Department of Microbiology, Columbia University, New York, New York 10032
^b Long Island University, Department of Biology, Brooklyn, New York 11201
^c Institut Curie, Paris, Cedex 75005, France
^d Marine Biological Laboratory, Woods Hole, Massachusetts 02543
Columbia University, 701 W. 168th St., Rm. 1404, New York, NY 10032.(212) 305-1468(212) 305-3930

pt143{at}columbia.edu

The correct positioning of the nucleus is often important indefining the spatial organization of the cell, for example,in determining the cell division plane. In interphase Schizosaccharomycespombe cells, the nucleus is positioned in the middle of thecylindrical cell in an active microtubule (MT)-dependent process.Here, we used green fluorescent protein markers to examine thedynamics of MTs, spindle pole body, and the nuclear envelopein living cells. We find that interphase MTs are organized inthree to four antiparallel MT bundles arranged along the longaxis of the cell, with MT plus ends facing both the cell tipsand minus ends near the middle of the cell. The MT bundles areorganized from medial MT-organizing centers that may functionas nuclear attachment sites. When MTs grow to the cell tips,they exert transient forces produced by plus end MT polymerizationthat push the nucleus. After an average of 1.5 min of growthat the cell tip, MT plus ends exhibit catastrophe and shrinkback to the nuclear region before growing back to the cell tip.Computer modeling suggests that a balance of these pushing MTforces can provide a mechanism to position the nucleus at themiddle of the cell.

Key Words: nuclear positioning • microtubule dynamics • microtubule-organizing center • green fluorescent protein • Schizosaccharomyces pombe

The online version of this paper contains supplemental material.

Abbreviations used in this paper: GFP, green fluorescent protein;iMTOC, interphase MTOC; MBL, methyl-2-benzimidazole-carbamate;MT, microtubule; MTOC, MT-organizing center; SPB, spindle polebody; TBZ, thiabendazole.

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