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Published online 16 April 2001. doi:10.1083/jcb.153.2.443
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© The Rockefeller University Press, 0021-9525/2001//443 $5.00
The Journal of Cell Biology, Volume 153, Number 2, , 2001 443-448

Report

Flagellar Radial Spoke Protein 3 Is an a-Kinase Anchoring Protein (Akap)



Anne Roush Gaillarda, Dennis R. Dienerb, Joel L. Rosenbaumb, and Winfield S. Salea

a Department of Cell Biology, Emory University School of Medicine, Atlanta, Georgia 30322
b Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520
Department of Cell Biology, Emory University School of Medicine, 1648 Pierce Dr., Atlanta, GA 30322.(404) 727-6256(404) 727-6265

win{at}cellbio.emory.edu

Previous physiological and pharmacological experiments have demonstrated that the Chlamydomonas flagellar axoneme contains a cAMP-dependent protein kinase (PKA) that regulates axonemal motility and dynein activity. However, the mechanism for anchoring PKA in the axoneme is unknown. Here we test the hypothesis that the axoneme contains an A-kinase anchoring protein (AKAP). By performing RII blot overlays on motility mutants defective for specific axonemal structures, two axonemal AKAPs have been identified: a 240-kD AKAP associated with the central pair apparatus, and a 97-kD AKAP located in the radial spoke stalk. Based on a detailed analysis, we have shown that AKAP97 is radial spoke protein 3 (RSP3). By expressing truncated forms of RSP3, we have localized the RII-binding domain to a region between amino acids 144–180. Amino acids 161–180 are homologous with the RII-binding domains of other AKAPs and are predicted to form an amphipathic helix. Amino acid substitution of the central residues of this region (L to P or VL to AA) results in the complete loss of RII binding. RSP3 is located near the inner arm dyneins, where an anchored PKA would be in direct position to modify dynein activity and regulate flagellar motility.

Key Words: kinases • cell motility • flagella • dynein • AKAP

© 2001 The Rockefeller University Press

Abbreviations used in this paper: AKAP, A-kinase anchoring protein; GST, glutathione-S-transferase; PKA, cAMP-dependent protein kinase; RSP, radial spoke protein.


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