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Published online 30 April 2001. doi:10.1083/jcb.153.3.449
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© The Rockefeller University Press, 0021-9525/2001//449 $5.00
The Journal of Cell Biology, Volume 153, Number 3, , 2001 449-456

Original Article

Aggresomes Resemble Sites Specialized for Virus Assembly



Colin M. Heatha, Miriam Windsora, and Thomas Wilemana

a Institute for Animal Health, Pirbright Laboratories, Surrey GU24 0NF, United Kingdom
Institute for Animal Health, Pirbright Laboratories, Ash Road, Woking, Surrey, England.44-1483-232-44844-1483-232-441

thomas.wileman{at}bbsrc.ac.uk

The large cytoplasmic DNA viruses such as poxviruses, iridoviruses, and African swine fever virus (ASFV) assemble in discrete perinuclear foci called viral factories. Factories exclude host proteins, suggesting that they are novel subcellular structures induced by viruses. Novel perinuclear structures, called aggresomes are also formed by cells in response to misfolded protein (Johnston, J.A., C.L. Ward, and R.R. Kopito. 1998. J. Cell Biol. 143:1883–1898; García-Mata, R., Z. Bebök, E.J. Sorscher, and E.S. Sztul. 1999. J. Cell Biol. 146:1239–1254). In this study, we have investigated whether aggresomes and viral factories are related structures. Aggresomes were compared with viral factories produced by ASFV. Aggresomes and viral factories were located close to the microtubule organizing center and required an intact microtubular network for assembly. Both structures caused rearrangement of intermediate filaments and the collapse of vimentin into characteristic cages, and both recruited mitochondria and cellular chaperones. Given that ASFV factories resemble aggresomes, it is possible that a cellular response originally designed to reduce the toxicity of misfolded proteins is exploited by cytoplasmic DNA viruses to concentrate structural proteins at virus assembly sites.

Key Words: virus assembly • aggresomes • African swine fever virus • microtubules • vimentin

© 2001 The Rockefeller University Press

The online version of this article contains supplemental material.

Dr. Heath's present address is Department of Cell Biology, Washington University Medical School, St. Louis, MO 63110.

Abbreviations used in this paper: ASFV, African swine fever virus; MTOC, microtubule organizing center.


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