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© The Rockefeller University Press, 0021-9525/2001//823 $5.00
The Journal of Cell Biology, Volume 153, Number 4, , 2001 823-834

A Central Role for the Armadillo Protein Plakoglobin in the Autoimmune Disease Pemphigus Vulgaris

^a Institute of Animal Pathology, University of Bern, CH-3012 Bern, Switzerland
^b Centre de Biologie du Developpement, 31062 Toulouse, France
Institute of Animal Pathology, Länggass-Strasse 122, 3012 Bern, Switzerland.41-31-631-26-3541-31-631-24-03 or 631-23-98

eliane.mueller{at}itpa.unibe.ch

In pemphigus vulgaris (PV), autoantibody binding to desmoglein (Dsg) 3 induces loss of intercellular adhesion in skin and mucous membranes. Two hypotheses are currently favored to explain the underlying molecular mechanisms: (a) disruption of adhesion through steric hindrance, and (b) interference of desmosomal cadherin-bound antibody with intracellular events, which we speculated to involve plakoglobin. To investigate the second hypothesis we established keratinocyte cultures from plakoglobin knockout (PG^–/–) embryos and PG^+/+ control mice. Although both cell types exhibited desmosomal cadherin-mediated adhesion during calcium-induced differentiation and bound PV immunoglobin (IgG) at their cell surface, only PG^+/+ keratinocytes responded with keratin retraction and loss of adhesion. When full-length plakoglobin was reintroduced into PG^–/– cells, responsiveness to PV IgG was restored. Moreover, in these cells like in PG^+/+ keratinocytes, PV IgG binding severely affected the linear distribution of plakoglobin at the plasma membrane. Taken together, the establishment of an in vitro model using PG^+/+ and PG^–/– keratinocytes allowed us (a) to exclude the steric hindrance only hypothesis, and (b) to demonstrate for the first time that plakoglobin plays a central role in PV, a finding that will provide a novel direction for investigations of the molecular mechanisms leading to PV, and on the function of plakoglobin in differentiating keratinocytes.

Key Words: catenins • desmosomes • mouse keratinocytes • epithelial differentiation • blistering disease

© 2001 The Rockefeller University Press

Abbreviations used in this paper: Dsg, desmoglein; E-cad, E-cadherin; GFP, green fluorescent protein; IF, immunofluorescence; nhIgG, normal human IgG; PV, pemphigus vulgaris; WB, Western blot.

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