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Published online 14 May 2001. doi:10.1083/jcb.153.4.835
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© The Rockefeller University Press, 0021-9525/2001//835 $5.00
The Journal of Cell Biology, Volume 153, Number 4, , 2001 835-850

Original Article

The Short Arm of the Laminin {gamma}2 Chain Plays a Pivotal Role in the Incorporation of Laminin 5 into the Extracellular Matrix and in Cell Adhesion



Laurent Gagnoux-Palaciosa, Maryline Allegraa, Flavia Spiritoa, Olivier Pommereta, Christine Romeroa, Jean-paul Ortonneb, and Guerrino Meneguzzia

a Faculty of Medicine, U385 Institut National de la Santé et de la Recherche Médicale, Cedex 2, 06107 Nice, France
b Dermatology Service, L'Archet Hospital, Cedex 3, 06202 Nice, France
U385 INSERM, Faculté de Médecine, Cedex 2, 06107 Nice, France.(33) 493-81-14-04(33) 493-37-77-78

meneguzz{at}unice.fr

Laminin 5 is a basement membrane component that actively promotes adhesion and migration of epithelial cells. Laminin 5 undergoes extracellular proteolysis of the {gamma}2 chain that removes the NH2-terminal short arm of the polypeptide and reduces the size of laminin 5 from 440 to 400 kD. The functional consequence of this event remains obscure, although lines of evidence indicate that cleavage of the {gamma}2 chain potently stimulated scattering and migration of keratinocytes and cancer cells. To define the biological role of the {gamma}2 chain short arm, we expressed mutated {gamma}2 cDNAs into immortalized {gamma}2-null keratinocytes. By immunofluorescence and immunohistochemical studies, cell detachment, and adhesion assays, we found that the {gamma}2 short arm drives deposition of laminin 5 into the extracellular matrix (ECM) and sustains cell adhesion. Our results demonstrate that the unprocessed 440-kD form of laminin 5 is a biologically active adhesion ligand, and that the {gamma}2 globular domain IV is involved in intermolecular interactions that mediate integration of laminin 5 in the ECM and cell attachment.

Key Words: keratinocyte • epithelial adhesion • cell migration • basement membrane • epidermolysis bullosa

© 2001 The Rockefeller University Press

Abbreviations used in this paper: ECM, extracellular matrix; HA, hemagglutinin; JEB, junctional epidermolysis bullosa; MMP, matrix metalloprotease; pAb, polyclonal antibody.


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