|
|
|
|
|
|
|
||
© The Rockefeller University Press,
0021-9525/2001//1085 $5.00
The Journal of Cell Biology, Volume 153, Number 5,
, 2001 1085-1096
Original Article |
Mba1, a Novel Component of the Mitochondrial Protein Export Machinery of the Yeast Saccharomyces cerevisiae
hannes.herrmann{at}bio.med.uni-muenchen.de
The biogenesis of mitochondria requires the integration of many proteins into the inner membrane from the matrix side. The inner membrane protein Oxa1 plays an important role in this process. We identified Mba1 as a second mitochondrial component that is required for efficient protein insertion. Like Oxa1, Mba1 specifically interacts both with mitochondrial translation products and with conservatively sorted, nuclear-encoded proteins during their integration into the inner membrane. Oxa1 and Mba1 overlap in function and substrate specificity, but both can act independently of each other. We conclude that Mba1 is part of the mitochondrial protein export machinery and represents the first component of a novel Oxa1-independent insertion pathway into the mitochondrial inner membrane.
Key Words: mitochondria • protein translocation • Mba1 • Oxa1 • membrane insertion
© 2001 The Rockefeller University Press
K. Hell's present address is Department of Biochemistry and Molecular Biology, Merck Frosst Research Laboratories, 16711 Autoroute Transcanadienne, Kirkland, Montréal, Québec H9H 3L1, Canada.R. Stuart's present address is Department of Biology, Marquette University, 530 N. 15th St., Milwaukee, WI 53233.
Abbreviations used in this paper: Cox, cytochrome oxidase; DFDNB, 1,5-difluoro-2,4-dinitrobenzene; DSG, disuccinimidyl glutarate; DSP, dithiobis(succinimidylpropinate); pCox, precursor Cox.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Facebook 
Reddit 
Technorati 
Twitter What's this?
|
|
