The Yeast Class V Myosins, Myo2p and Myo4p, Are Nonprocessive Actin-based Motors

doi:10.1083/jcb.153.5.1121

Published online 29 May 2001. doi:10.1083/jcb.153.5.1121

This Article

	Full Text
	Full Text (PDF, 118K)
	PPT slides of all figures
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Reck-Peterson, S. L.
	Articles by Mooseker, M. S.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Reck-Peterson, S. L.
	Articles by Mooseker, M. S.


Social Bookmarking

	What's this?

© The Rockefeller University Press, 0021-9525/2001/5/1121/ $5.00
The Journal of Cell Biology, Volume 153, Number 5, May 28, 2001 1121-1126

Report

The Yeast Class V Myosins, Myo2p and Myo4p, Are Nonprocessive Actin-based Motors

Samara L. Reck-Peterson^a,b, Matthew J. Tyska^a, Peter J. Novick^b, and Mark S. Mooseker^a,b,c
^a Department of Molecular, Cellular, and Developmental Biology,
^b Department of Cell Biology, Yale University, New Haven, Connecticut 06520
^c Department of Pathology, Yale University, New Haven, Connecticut 06520

Correspondence to: Mark S. Mooseker, Department of Molecular, Cellular, and Developmental Biology, Yale University, KBT 352, P.O. Box 208103, New Haven, CT 06520-8103. Tel:(203) 431-3468 Fax:(203) 432-6161 E-mail:mark.mooseker{at}yale.edu.

The motor properties of the two yeast class V myosins, Myo2pand Myo4p, were examined using in vitro motility assays. Bothmyosins are active motors with maximum velocities of 4.5 µm/sfor Myo2p and 1.1 µm/s for Myo4p. Myo2p motility is Ca²⁺insensitive. Both myosins have properties of a nonprocessivemotor, unlike chick myosin-Va (M5a), which behaves as a processivemotor when assayed under identical conditions. Additional supportfor the idea that Myo2p is a nonprocessive motor comes fromactin cosedimentation assays, which show that Myo2p has a lowaffinity for F-actin in the presence of ATP and Ca²⁺, unlikechick brain M5a. These studies suggest that if Myo2p functionsin organelle transport, at least five molecules of Myo2p mustbe present per organelle to promote directed movement.

Key Words: myosin-V, cytoskeleton, in vitro motility, processive motor,cerevisiae

Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

Taft, M. H., Hartmann, F. K., Rump, A., Keller, H., Chizhov, I., Manstein, D. J., Tsiavaliaris, G. (2008). Dictyostelium Myosin-5b Is a Conditional Processive Motor. J. Biol. Chem. 283: 26902-26910 [Abstract] [Full Text]
Watanabe, S., Watanabe, T. M., Sato, O., Awata, J., Homma, K., Umeki, N., Higuchi, H., Ikebe, R., Ikebe, M. (2008). Human Myosin Vc Is a Low Duty Ratio Nonprocessive Motor. J. Biol. Chem. 283: 10581-10592 [Abstract] [Full Text]
Hodges, A. R., Krementsova, E. B., Trybus, K. M. (2008). She3p Binds to the Rod of Yeast Myosin V and Prevents It from Dimerizing, Forming a Single-headed Motor Complex. J. Biol. Chem. 283: 6906-6914 [Abstract] [Full Text]
Chang, W., Zaarour, R. F., Reck-Peterson, S., Rinn, J., Singer, R. H., Snyder, M., Novick, P., Mooseker, M. S. (2008). Myo2p, a class V myosin in budding yeast, associates with a large ribonucleic acid-protein complex that contains mRNAs and subunits of the RNA-processing body. RNA 14: 491-502 [Abstract] [Full Text]
Heuck, A., Du, T.-G., Jellbauer, S., Richter, K., Kruse, C., Jaklin, S., Muller, M., Buchner, J., Jansen, R.-P., Niessing, D. (2007). Monomeric myosin V uses two binding regions for the assembly of stable translocation complexes. Proc. Natl. Acad. Sci. USA 104: 19778-19783 [Abstract] [Full Text]
Szczesna-Cordary, D., Jones, M., Moore, J. R., Watt, J., Kerrick, W. G. L., Xu, Y., Wang, Y., Wagg, C., Lopaschuk, G. D. (2007). Myosin regulatory light chain E22K mutation results in decreased cardiac intracellular calcium and force transients. FASEB J. 21: 3974-3985 [Abstract] [Full Text]
Grallert, A., Martin-Garcia, R., Bagley, S., Mulvihill, D. P. (2007). In vivo movement of the type V myosin Myo52 requires dimerisation but is independent of the neck domain. J. Cell Sci. 120: 4093-4098 [Abstract] [Full Text]
Dunn, B. D., Sakamoto, T., Hong, M.-S. S., Sellers, J. R., Takizawa, P. A. (2007). Myo4p is a monomeric myosin with motility uniquely adapted to transport mRNA. JCB 178: 1193-1206 [Abstract] [Full Text]
Hachikubo, Y., Ito, K., Schiefelbein, J., Manstein, D. J., Yamamoto, K. (2007). Enzymatic Activity and Motility of Recombinant Arabidopsis Myosin XI, MYA1. Plant Cell Physiol 48: 886-891 [Abstract] [Full Text]
Steinberg, G. (2007). Hyphal Growth: a Tale of Motors, Lipids, and the Spitzenkorper. Eukaryot Cell 6: 351-360 [Full Text]
Huckaba, T. M., Lipkin, T., Pon, L. A. (2006). Roles of type II myosin and a tropomyosin isoform in retrograde actin flow in budding yeast. JCB 175: 957-969 [Abstract] [Full Text]
Olivares, A. O., Chang, W., Mooseker, M. S., Hackney, D. D., De La Cruz, E. M. (2006). The Tail Domain of Myosin Va Modulates Actin Binding to One Head. J. Biol. Chem. 281: 31326-31336 [Abstract] [Full Text]
Moseley, J. B., Goode, B. L. (2006). The Yeast Actin Cytoskeleton: from Cellular Function to Biochemical Mechanism. Microbiol. Mol. Biol. Rev. 70: 605-645 [Abstract] [Full Text]
Krementsova, E. B., Hodges, A. R., Lu, H., Trybus, K. M. (2006). Processivity of Chimeric Class V Myosins. J. Biol. Chem. 281: 6079-6086 [Abstract] [Full Text]
Shih, J. L., Reck-Peterson, S. L., Newitt, R., Mooseker, M. S., Aebersold, R., Herskowitz, I. (2005). Cell Polarity Protein Spa2P Associates With Proteins Involved In Actin Function In Saccharomyces Cerevisiae. Mol. Biol. Cell 16: 4595-4608 [Abstract] [Full Text]
Terrak, M., Rebowski, G., Lu, R. C., Grabarek, Z., Dominguez, R. (2005). Structure of the light chain-binding domain of myosin V. Proc. Natl. Acad. Sci. USA 102: 12718-12723 [Abstract] [Full Text]
Toth, J., Kovacs, M., Wang, F., Nyitray, L., Sellers, J. R. (2005). Myosin V from Drosophila Reveals Diversity of Motor Mechanisms within the Myosin V Family. J. Biol. Chem. 280: 30594-30603 [Abstract] [Full Text]
Krendel, M., Mooseker, M. S. (2005). Myosins: Tails (and Heads) of Functional Diversity. Physiology 20: 239-251 [Abstract] [Full Text]
Gonsalvez, G. B., Little, J. L., Long, R. M. (2004). ASH1 mRNA Anchoring Requires Reorganization of the Myo4p-She3p-She2p Transport Complex. J. Biol. Chem. 279: 46286-46294 [Abstract] [Full Text]
Estrada, P., Kim, J., Coleman, J., Walker, L., Dunn, B., Takizawa, P., Novick, P., Ferro-Novick, S. (2003). Myo4p and She3p are required for cortical ER inheritance in Saccharomyces cerevisiae. JCB 163: 1255-1266 [Abstract] [Full Text]
Vale, R. D. (2003). Myosin V motor proteins: marching stepwise towards a mechanism. JCB 163: 445-450 [Abstract] [Full Text]
GONSALVEZ, G. B., LEHMANN, K. A., HO, D. K., STANITSA, E. S., WILLIAMSON, J. R., LONG, R. M. (2003). RNA-protein interactions promote asymmetric sorting of the ASH1 mRNA ribonucleoprotein complex. RNA 9: 1383-1399 [Abstract] [Full Text]
Toi, H., Fujimura-Kamada, K., Irie, K., Takai, Y., Todo, S., Tanaka, K. (2003). She4p/Dim1p Interacts with the Motor Domain of Unconventional Myosins in the Budding Yeast, Saccharomyces cerevisiae. Mol. Biol. Cell 14: 2237-2249 [Abstract] [Full Text]
Bretscher, A. (2003). Polarized growth and organelle segregation in yeast: the tracks, motors, and receptors. JCB 160: 811-816 [Abstract] [Full Text]
Kruse, C., Jaedicke, A., Beaudouin, J., Bohl, F., Ferring, D., Guttler, T., Ellenberg, J., Jansen, R.-P. (2002). Ribonucleoprotein-dependent localization of the yeast class V myosin Myo4p. JCB 159: 971-982 [Abstract] [Full Text]
Post, P. L., Tyska, M. J., O'Connell, C. B., Johung, K., Hayward, A., Mooseker, M. S. (2002). Myosin-IXb Is a Single-headed and Processive Motor. J. Biol. Chem. 277: 11679-11683 [Abstract] [Full Text]
Tauhata, S. B. F., dos Santos, D. V., Taylor, E. W., Mooseker, M. S., Larson, R. E. (2001). High Affinity Binding of Brain Myosin-Va to F-actin Induced by Calcium in the Presence of ATP. J. Biol. Chem. 276: 39812-39818 [Abstract] [Full Text]