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Published online 11 June 2001. doi:10.1083/jcb.153.6.1227
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© The Rockefeller University Press, 0021-9525/2001//1227 $5.00
The Journal of Cell Biology, Volume 153, Number 6, , 2001 1227-1238

Original Article

Him-10 Is Required for Kinetochore Structure and Function on Caenorhabditis elegans Holocentric Chromosomes



Mary Howea, Kent L. McDonaldb, Donna G. Albertsonc, and Barbara J. Meyera

a Howard Hughes Medical Institute, Department of Molecular and Cell Biology
b Electron Microscope Laboratory, University of California, Berkeley, Berkeley, California 94720
c Cancer Research Institute, University of California, San Francisco, San Francisco, California 94143
Howard Hughes Medical Institute, Department of Molecular and Cell Biology, 401 Barker Hall, University of California, Berkeley, Berkeley, CA 94720-3204.(510) 643-5584(510) 643-5585

bjmeyer{at}uclink4.berkeley.edu

Macromolecular structures called kinetochores attach and move chromosomes within the spindle during chromosome segregation. Using electron microscopy, we identified a structure on the holocentric mitotic and meiotic chromosomes of Caenorhabditis elegans that resembles the mammalian kinetochore. This structure faces the poles on mitotic chromosomes but encircles meiotic chromosomes. Worm kinetochores require the evolutionarily conserved HIM-10 protein for their structure and function. HIM-10 localizes to the kinetochores and mediates attachment of chromosomes to the spindle. Depletion of HIM-10 disrupts kinetochore structure, causes a failure of bipolar spindle attachment, and results in chromosome nondisjunction. HIM-10 is related to the Nuf2 kinetochore proteins conserved from yeast to humans. Thus, the extended kinetochores characteristic of C. elegans holocentric chromosomes provide a guide to the structure, molecular architecture, and function of conventional kinetochores.

Key Words: kinetochore • holocentric chromosomes • mitosis • meiosis • Caenorhabditis elegans

© 2001 The Rockefeller University Press

Abbreviations used in this paper: FS, freeze substitution; HPF, high pressure freezing; RNAi, RNA-mediated interference; Unc, uncoordinated.


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