Targeting of an abundant cytosolic form of the protein import receptor at Toc159 to the outer chloroplast membrane

doi:10.1083/jcb.200104022

Published 23 July 2001. doi:10.1083/jcb.200104022

This Article

Full Text

Full Text (PDF, 269K)

PPT slides of all figures

Alert me when this article is cited

Citation Map

Services

Email this article

Similar articles in this journal

Similar articles in PubMed

Alert me to new content in the JCB

Download to citation manager

Citing Articles

Citing Articles via HighWire

Citing Articles via CrossRef

Citing Articles via Google Scholar

Google Scholar

Articles by Hiltbrunner, A.

Articles by Kessler, F.

Search for Related Content

PubMed

PubMed Citation

Articles by Hiltbrunner, A.

Articles by Kessler, F.

Pubmed/NCBI databases

Domain

Social Bookmarking

What's this?

© The Rockefeller University Press, 0021-9525/2001/7/309 $5.00
The Journal of Cell Biology, Volume 154, Number 2, July 23, 2001 309-316

Article

Targeting of an abundant cytosolic form of the protein import receptor at Toc159 to the outer chloroplast membrane

Andreas Hiltbrunner¹, Jörg Bauer¹, Pierre-Alexandre Vidi¹, Sibylle Infanger¹, Petra Weibel¹, Morten Hohwy² and Felix Kessler¹

¹ Institute of Plant Sciences, Plant Physiology and Biochemistry Group, 8092 Zürich, Switzerland
² Institute of Molecular Biology and Biophysics, 8093 Zürich, Switzerland

Address correspondence to Felix Kessler, Institute of Plant Sciences, Plant Physiology and Biochemistry Group, ETH Zürich, Universitätstrasse 2, 8092 Zürich, Switzerland. Tel.: 41-1-632-60-15. Fax: 41-1-632-10-84. E-mail: felix.kessler{at}ipw.biol.ethz.ch

Chloroplast biogenesis requires the large-scale import of cytosolicallysynthesized precursor proteins. A trimeric translocon (Toc complex)containing two homologous GTP-binding proteins (atToc33 andatToc159) and a channel protein (atToc75) facilitates proteintranslocation across the outer envelope membrane. The mechanismsgoverning function and assembly of the Toc complex are not yetunderstood. This study demonstrates that atToc159 and its peaorthologue exist in an abundant, previously unrecognized solubleform, and partition between cytosol-containing soluble fractionsand the chloroplast outer membrane. We show that soluble atToc159binds directly to the cytosolic domain of atToc33 in a homotypicinteraction, contributing to the integration of atToc159 intothe chloroplast outer membrane. The data suggest that the functionof the Toc complex involves switching of atToc159 between asoluble and an integral membrane form.

Key Words: chloroplasts; protein import; translocon; complex assembly; soluble receptor

Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

This article has been cited by other articles:

Lee, J., Wang, F., Schnell, D. J. (2009). Toc Receptor Dimerization Participates in the Initiation of Membrane Translocation during Protein Import into Chloroplasts. J. Biol. Chem. 284: 31130-31141 [Abstract] [Full Text]
Jouhet, J., Gray, J. C. (2009). Interaction of Actin and the Chloroplast Protein Import Apparatus. J. Biol. Chem. 284: 19132-19141 [Abstract] [Full Text]
Agne, B., Infanger, S., Wang, F., Hofstetter, V., Rahim, G., Martin, M., Lee, D. W., Hwang, I., Schnell, D., Kessler, F. (2009). A Toc159 Import Receptor Mutant, Defective in Hydrolysis of GTP, Supports Preprotein Import into Chloroplasts. J. Biol. Chem. 284: 8670-8679 [Abstract] [Full Text]
Rahim, G., Bischof, S., Kessler, F., Agne, B. (2009). In vivo interaction between atToc33 and atToc159 GTP-binding domains demonstrated in a plant split-ubiquitin system. J Exp Bot 60: 257-267 [Abstract] [Full Text]
Koenig, P., Oreb, M., Rippe, K., Muhle-Goll, C., Sinning, I., Schleiff, E., Tews, I. (2008). On the Significance of Toc-GTPase Homodimers. J. Biol. Chem. 283: 23104-23112 [Abstract] [Full Text]
Miras, S., Salvi, D., Piette, L., Seigneurin-Berny, D., Grunwald, D., Reinbothe, C., Joyard, J., Reinbothe, S., Rolland, N. (2007). Toc159- and Toc75-independent Import of a Transit Sequence-less Precursor into the Inner Envelope of Chloroplasts. J. Biol. Chem. 282: 29482-29492 [Abstract] [Full Text]
Yeh, Y.-H., Kesavulu, M. M., Li, H.-m., Wu, S.-Z., Sun, Y.-J., Konozy, E. H. E., Hsiao, C.-D. (2007). Dimerization Is Important for the GTPase Activity of Chloroplast Translocon Components atToc33 and psToc159. J. Biol. Chem. 282: 13845-13853 [Abstract] [Full Text]
Nakayama, K., Okawa, K., Kakizaki, T., Honma, T., Itoh, H., Inaba, T. (2007). Arabidopsis Cor15am Is a Chloroplast Stromal Protein That Has Cryoprotective Activity and Forms Oligomers. Plant Physiol. 144: 513-523 [Abstract] [Full Text]
Reddick, L. E., Vaughn, M. D., Wright, S. J., Campbell, I. M., Bruce, B. D. (2007). In Vitro Comparative Kinetic Analysis of the Chloroplast Toc GTPases. J. Biol. Chem. 282: 11410-11426 [Abstract] [Full Text]
Vidi, P.-A., Kanwischer, M., Baginsky, S., Austin, J. R., Csucs, G., Dormann, P., Kessler, F., Brehelin, C. (2006). Tocopherol Cyclase (VTE1) Localization and Vitamin E Accumulation in Chloroplast Plastoglobule Lipoprotein Particles. J. Biol. Chem. 281: 11225-11234 [Abstract] [Full Text]
Bedard, J., Jarvis, P. (2005). Recognition and envelope translocation of chloroplast preproteins. J Exp Bot 56: 2287-2320 [Abstract] [Full Text]
Baldwin, A., Wardle, A., Patel, R., Dudley, P., Park, S. K., Twell, D., Inoue, K., Jarvis, P. (2005). A Molecular-Genetic Study of the Arabidopsis Toc75 Gene Family. Plant Physiol. 138: 715-733 [Abstract] [Full Text]
Ko, K., Taylor, D., Argenton, P., Innes, J., Pedram, B., Seibert, F., Granell, A., Ko, Z. (2005). Evidence That the Plastid Translocon Tic40 Components Possess Modulating Capabilities. J. Biol. Chem. 280: 215-224 [Abstract] [Full Text]
Kubis, S., Patel, R., Combe, J., Bedard, J., Kovacheva, S., Lilley, K., Biehl, A., Leister, D., Rios, G., Koncz, C., Jarvis, P. (2004). Functional Specialization amongst the Arabidopsis Toc159 Family of Chloroplast Protein Import Receptors. Plant Cell 16: 2059-2077 [Abstract] [Full Text]
Ivanova, Y., Smith, M. D., Chen, K., Schnell, D. J. (2004). Members of the Toc159 Import Receptor Family Represent Distinct Pathways for Protein Targeting to Plastids. Mol. Biol. Cell 15: 3379-3392 [Abstract] [Full Text]
Smith, M. D., Rounds, C. M., Wang, F., Chen, K., Afitlhile, M., Schnell, D. J. (2004). atToc159 is a selective transit peptide receptor for the import of nucleus-encoded chloroplast proteins. JCB 165: 323-334 [Abstract] [Full Text]
Wallas, T. R., Smith, M. D., Sanchez-Nieto, S., Schnell, D. J. (2003). The Roles of Toc34 and Toc75 in Targeting the Toc159 Preprotein Receptor to Chloroplasts. J. Biol. Chem. 278: 44289-44297 [Abstract] [Full Text]
Inaba, T., Li, M., Alvarez-Huerta, M., Kessler, F., Schnell, D. J. (2003). atTic110 Functions as a Scaffold for Coordinating the Stromal Events of Protein Import into Chloroplasts. J. Biol. Chem. 278: 38617-38627 [Abstract] [Full Text]
Weibel, P., Hiltbrunner, A., Brand, L., Kessler, F. (2003). Dimerization of Toc-GTPases at the Chloroplast Protein Import Machinery. J. Biol. Chem. 278: 37321-37329 [Abstract] [Full Text]
Lee, K. H., Kim, S. J., Lee, Y. J., Jin, J. B., Hwang, I. (2003). The M Domain of atToc159 Plays an Essential Role in the Import of Proteins into Chloroplasts and Chloroplast Biogenesis. J. Biol. Chem. 278: 36794-36805 [Abstract] [Full Text]
Kubis, S., Baldwin, A., Patel, R., Razzaq, A., Dupree, P., Lilley, K., Kurth, J., Leister, D., Jarvis, P. (2003). The Arabidopsis ppi1 Mutant Is Specifically Defective in the Expression, Chloroplast Import, and Accumulation of Photosynthetic Proteins. Plant Cell 15: 1859-1871 [Abstract] [Full Text]
Schleiff, E., Jelic, M., Soll, J. (2003). A GTP-driven motor moves proteins across the outer envelope of chloroplasts. Proc. Natl. Acad. Sci. USA 100: 4604-4609 [Abstract] [Full Text]
Qbadou, S., Tien, R., Soll, J., Schleiff, E. (2003). Membrane insertion of the chloroplast outer envelope protein, Toc34: constrains for insertion and topology. J. Cell Sci. 116: 837-846 [Abstract] [Full Text]
Schleiff, E., Soll, J., Kuchler, M., Kuhlbrandt, W., Harrer, R. (2003). Characterization of the translocon of the outer envelope of chloroplasts. JCB 160: 541-551 [Abstract] [Full Text]
Bauer, J., Hiltbrunner, A., Weibel, P., Vidi, P.-A., Alvarez-Huerta, M., Smith, M. D., Schnell, D. J., Kessler, F. (2002). Essential role of the G-domain in targeting of the protein import receptor atToc159 to the chloroplast outer membrane. JCB 159: 845-854 [Abstract] [Full Text]
Smith, M. D., Hiltbrunner, A., Kessler, F., Schnell, D. J. (2002). The targeting of the atToc159 preprotein receptor to the chloroplast outer membrane is mediated by its GTPase domain and is regulated by GTP. JCB 159: 833-843 [Abstract] [Full Text]