JCB logo
Accuri Cytometers
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published 23 July 2001. doi:10.1083/jcb.200104073
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 1245K)
Right arrow PPT slides of all figures
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jokitalo, E.
Right arrow Articles by Shima, D. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jokitalo, E.
Right arrow Articles by Shima, D. T.
Right arrowPubmed/NCBI databases
*Substance via MeSH
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

© The Rockefeller University Press, 0021-9525/2001/7/317 $5.00
The Journal of Cell Biology, Volume 154, Number 2, July 23, 2001 317-330

Article

Golgi clusters and vesicles mediate mitotic inheritance independently of the endoplasmic reticulum



Eija Jokitalo1, Noemi Cabrera-Poch2, Graham Warren3 and David T. Shima4

1 Institute of Biotechnology, Electron Microscopy Unit, University of Helsinki, 00014 Helsinki, Finland
2 Department of Anatomy and Cell Biology, Columbia University, College of Physicians and Surgeons, New York, NY 10032
3 Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520
4 Endothelial Cell Biology Laboratory, Imperial Cancer Research Fund, London WC2A 3PX, UK

Address correspondence to David T. Shima, Endothelial Cell Biology Laboratory, Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, London WC2A 3PX, UK. Tel.: 44-207-269-2880. Fax: 44-207-269-3417. E-mail: d.shima{at}magic.lif.icnet.uk

We have examined the fate of Golgi membranes during mitotic inheritance in animal cells using four-dimensional fluorescence microscopy, serial section reconstruction of electron micrographs, and peroxidase cytochemistry to track the fate of a Golgi enzyme fused to horseradish peroxidase. All three approaches show that partitioning of Golgi membranes is mediated by Golgi clusters that persist throughout mitosis, together with shed vesicles that are often found associated with spindle microtubules. We have been unable to find evidence that Golgi membranes fuse during the later phases of mitosis with the endoplasmic reticulum (ER) as a strategy for Golgi partitioning (Zaal, K.J., C.L. Smith, R.S. Polishchuk, N. Altan, N.B. Cole, J. Ellenberg, K. Hirschberg, J.F. Presley, T.H. Roberts, E. Siggia, et al. 1999. Cell. 99:589–601) and suggest that these results, in part, are the consequence of slow or abortive folding of GFP–Golgi chimeras in the ER. Furthermore, we show that accurate partitioning is accomplished early in mitosis, by a process of cytoplasmic redistribution of Golgi fragments and vesicles yielding a balance of Golgi membranes on either side of the metaphase plate before cell division.

Key Words: Golgi apparatus; mitosis; endoplasmic reticulum; organelle inheritance; vesicle


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents