S-Nitrosylation of mitochondrial caspases

doi:10.1083/jcb.200104008

Published online 10 September 2001. doi:10.1083/jcb.200104008

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© The Rockefeller University Press, 0021-9525/2001/9/1111 $5.00
The Journal of Cell Biology, Volume 154, Number 6, September 17, 2001 1111-1116

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S-Nitrosylation of mitochondrial caspases

Joan B. Mannick¹, Christopher Schonhoff¹, Natalia Papeta², Pedram Ghafourifar¹, Marten Szibor¹, Kezhong Fang³ and Benjamin Gaston³

¹ Department of Medicine, University of Massachusetts Medical School, Shrewsbury, MA 01545
² Department of Medicine, Beth Israel Hospital, Boston, MA 02115
³ Department of Pediatrics, University of Virginia Health Sciences Center, Charlottesville, VA 22908

Address correspondence to Joan B. Mannick, Dept. of Medicine, University of Massachusetts Medical School, Rose Reed Gordon Bldg., 222 Maple Ave., Shrewsbury, MA 01545. Tel.: (508) 856-7511. Fax: (508) 856-7578. E-mail: joan.mannick{at}umassmed.edu

Caspase-3 is a cysteine protease located in both the cytoplasmand mitochondrial intermembrane space that is a central effectorof many apoptotic pathways. In resting cells, a subset of caspase-3zymogens is S-nitrosylated at the active site cysteine, inhibitingenzyme activity. During Fas-induced apoptosis, caspases aredenitrosylated, allowing the catalytic site to function. Inthe current studies, we sought to identify the subpopulationof caspases that is regulated by S-nitrosylation. We reportthat the majority of mitochondrial, but not cytoplasmic, caspase-3zymogens contain this inhibitory modification. In addition,the majority of mitochondrial caspase-9 is S-nitrosylated. Thesestudies suggest that S-nitrosylation plays an important rolein regulating mitochondrial caspase function and that the S-nitrosylationstate of a given protein depends on its subcellular localization.

Key Words: nitric oxide; caspase-3; caspase-9; mitochondria; S-nitrosylation

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