Generation of high curvature membranes mediated by direct endophilin bilayer interactions

doi:10.1083/jcb.200107075

Published 15 October 2001. doi:10.1083/jcb.200107075

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© The Rockefeller University Press, 0021-9525/2001/10/193 $5.00
The Journal of Cell Biology, Volume 155, Number 2, October 15, 2001 193-200

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Generation of high curvature membranes mediated by direct endophilin bilayer interactions

Khashayar Farsad, Niels Ringstad, Kohji Takei, Scott R. Floyd, Kristin Rose and Pietro De Camilli

Howard Hughes Medical Institute and Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06511

Address correspondence to Dr. Pietro De Camilli, Department of Cell Biology, Howard Hughes Medical Institute, Boyer Center for Molecular Medicine, Yale University School of Medicine, 295 Congress Ave., New Haven, CT 06510. Tel.: (203) 737-4465. Fax: (203) 737-4436. E-mail: pietro.decamilli{at}yale.edu

Endophilin 1 is a presynaptically enriched protein which bindsthe GTPase dynamin and the polyphosphoinositide phosphatasesynptojanin. Perturbation of endophilin function in cell-freesystems and in a living synapse has implicated endophilin inendocytic vesicle budding (Ringstad, N., H. Gad, P. Low, G.Di Paolo, L. Brodin, O. Shupliakov, and P. De Camilli. 1999.Neuron. 24:143–154; Schmidt, A., M. Wolde, C. Thiele,W. Fest, H. Kratzin, A.V. Podtelejnikov, W. Witke, W.B. Huttner,and H.D. Soling. 1999. Nature. 401:133–141; Gad, H., N.Ringstad, P. Low, O. Kjaerulff, J. Gustafsson, M. Wenk, G. DiPaolo, Y. Nemoto, J. Crun, M.H. Ellisman, et al. 2000. Neuron.27:301–312). Here, we show that purified endophilin candirectly bind and evaginate lipid bilayers into narrow tubulessimilar in diameter to the neck of a clathrin-coated bud, providingnew insight into the mechanisms through which endophilin mayparticipate in membrane deformation and vesicle budding. Thisproperty of endophilin is independent of its putative lysophosphatydicacid acyl transferase activity, is mediated by its NH₂-terminalregion, and requires an amino acid stretch homologous to a correspondingregion in amphiphysin, a protein previously shown to have similareffects on lipid bilayers (Takei, K., V.I. Slepnev, V. Haucke,and P. De Camilli. 1999. Nat. Cell Biol. 1:33–39). Endophilincooligomerizes with dynamin rings on lipid tubules and inhibitsdynamin's GTP-dependent vesiculating activity. Endophilin B,a protein with homology to endophilin 1, partially localizesto the Golgi complex and also deforms lipid bilayers into tubules,underscoring a potential role of endophilin family members indiverse tubulovesicular membrane-trafficking events in the cell.

Key Words: SH3P4; dynamin; endocytosis; RICH; endophilin B1

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