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© The Rockefeller University Press,
0021-9525/2001/11/625 $5.00
The Journal of Cell Biology, Volume 155, Number 4, November 12, 2001 625-636
Article |
Myosin V exhibits a high duty cycle and large unitary displacement
Address correspondence to David M. Warshaw, Molecular Physiology and Biophysics, University of Vermont, Burlington, VT 05405. Tel.: (802) 656-4300. Fax: (802) 656-0747. E-mail: warshaw{at}physiology.med.uvm.edu
Myosin V is a double-headed unconventional myosin that has been implicated in organelle transport. To perform this role, myosin V may have a high duty cycle. To test this hypothesis and understand the properties of this molecule at the molecular level, we used the laser trap and in vitro motility assay to characterize the mechanics of heavy meromyosin–like fragments of myosin V (M5HMM) expressed in the Baculovirus system. The relationship between actin filament velocity and the number of interacting M5HMM molecules indicates a duty cycle of
50%. This high duty cycle would allow actin filament translocation and thus organelle transport by a few M5HMM molecules. Single molecule displacement data showed predominantly single step events of 20 nm and an occasional second step to 37 nm. The 20-nm unitary step represents the myosin V working stroke and is independent of the mode of M5HMM attachment to the motility surface or light chain content. The large M5HMM working stroke is consistent with the myosin V neck acting as a mechanical lever. The second step is characterized by an increased displacement variance, suggesting a model for how the two heads of myosin V function in processive motion.
Key Words: myosin V; in vitro motility; laser trap; single molecule; mechanics
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J. Cell Biol. 2001 155: 495.
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