JCB logo
Avanti Polar Lipids, Inc.
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published 12 November 2001. doi:10.1083/jcb.200108080
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 334K)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hoffmann, P. R.
Right arrow Articles by Henson, P. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hoffmann, P. R.
Right arrow Articles by Henson, P. M.
Related Collections
Right arrowRelated Article
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

© The Rockefeller University Press, 0021-9525/2001/11/649 $5.00
The Journal of Cell Biology, Volume 155, Number 4, November 12, 2001 649-660

Article

Phosphatidylserine (PS) induces PS receptor–mediated macropinocytosis and promotes clearance of apoptotic cells



Peter R. Hoffmann1, Aimee M. deCathelineau1, Carol Anne Ogden1, Yann Leverrier2, Donna L. Bratton1, David L. Daleke3, Anne J. Ridley2, Valerie A. Fadok1 and Peter M. Henson1

1 Program in Cell Biology, Department of Pediatrics, National Jewish Medical and Research Center, Denver, CO 80206
2 Ludwig Institute for Cancer Research, London W1P 8BT, UK
3 Department of Biochemistry and Molecular Biology/Medical Sciences, Indiana University, Bloomington, IN 47405

Address correspondence to Peter Henson, National Jewish Medical and Research Center, D508, 1400 Jackson Street, Denver, CO 80206. Tel.: (303) 398-1380. Fax: (303) 398-1381. E-mail: hensonp{at}njc.org

Efficient phagocytosis of apoptotic cells is important for normal tissue development, homeostasis, and the resolution of inflammation. Although many receptors have been implicated in the clearance of apoptotic cells, the roles of these receptors in the engulfment process have not been well defined. We developed a novel system to distinguish between receptors involved in tethering of apoptotic cells versus those inducing their uptake. Our results suggest that regardless of the receptors engaged on the phagocyte, ingestion does not occur in the absence of phosphatidylserine (PS). Further, recognition of PS was found to be dependent on the presence of the PS receptor (PSR). Both PS and anti-PSR antibodies stimulated membrane ruffling, vesicle formation, and "bystander" uptake of cells bound to the surface of the phagocyte. We propose that the phagocytosis of apoptotic cells requires two events: tethering followed by PS-stimulated, PSR-mediated macropinocytosis.

Key Words: macropinocytosis; phagocytosis; apoptotic cells; phosphatidylserine; phosphatidylserine receptor


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?

Related Article

A marker for munching
William A. Wells
J. Cell Biol. 2001 155: 495. [Full Text] [PDF]



This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents