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© The Rockefeller University Press, 0021-9525/2001/11/711 $5.00
The Journal of Cell Biology, Volume 155, Number 5, November 26, 2001 711-718

Correct spindle elongation at the metaphase/anaphase transition is an APC-dependent event in budding yeast

¹ Max Planck Institute for Cell Biology and Genetics, Dresden 01307, Germany
² Dipartimento di Biotecnologie e Bioscienze, Universitá degli Studi di Milano-Bicocca, 20126 Milano, Italy

Address correspondence to Simonetta Piatti, Dipartimento di Biotecnologie e Bioscienze, Piazza della Scienza 2, 20126 Milano, Italy. Tel.: 39-02-6448-3547. Fax: 39-02-6448-3565. E-mail: simonetta.piatti{at}unimib.it

At the metaphase to anaphase transition, chromosome segregation is initiated by the splitting of sister chromatids. Subsequently, spindles elongate, separating the sister chromosomes into two sets. Here, we investigate the cell cycle requirements for spindle elongation in budding yeast using mutants affecting sister chromatid cohesion or DNA replication. We show that separation of sister chromatids is not sufficient for proper spindle integrity during elongation. Rather, successful spindle elongation and stability require both sister chromatid separation and anaphase-promoting complex activation. Spindle integrity during elongation is dependent on proteolysis of the securin Pds1 but not on the activity of the separase Esp1. Our data suggest that stabilization of the elongating spindle at the metaphase to anaphase transition involves Pds1-dependent targets other than Esp1.

Key Words: anaphase; anaphase-promoting complex; microtubules; securin; spindle

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