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Published online 19 November 2001. doi:10.1083/jcb.200106044
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© The Rockefeller University Press, 0021-9525/2001/11/719 $5.00
The Journal of Cell Biology, Volume 155, Number 5, November 26, 2001 719-724

Report

NAPO as a novel marker for apoptosis



Berna S. Sayan1, Gulayse Ince1, A. Emre Sayan1 and Mehmet Ozturk1,2

1 Department of Molecular Biology, Bilkent University, 06533 Ankara, Turkey
2 BilGen Genetics and Biotechnology Center, Bilkent University, 06533 Ankara, Turkey

Address correspondence to Mehmet Ozturk, Department of Molecular Biology and Genetics, Bilkent University, 06533 Ankara, Turkey. Tel.: (90) 312-266-50-81. Fax: (90) 312-266-50-97. E-mail: ozturk{at}fen.bilkent.edu.tr

Apoptosis or programmed cell death plays a pivotal role in embryonic development and maintenance of homeostasis. It is also involved in the etiology of pathophysiological conditions such as cancer, neurodegenerative, autoimmune, infectious, and heart diseases. Consequently, the study of apoptosis is now at center of both basic and clinical research applications. Therefore, sensitive and simple apoptosis detection techniques are required. Here we describe a monoclonal antibody–defined novel antigen, namely NAPO (negative in apoptosis), which is specifically lost during apoptosis. The anti-NAPO antibody recognizes two nuclear polypeptides of 60 and 70 kD. The antigen is maintained in quiescent and senescent cells, as well as in different phases of the cell cycle, including mitosis. Thus, immunodetection of NAPO antigen provides a specific, sensitive, and easy method for differential identification of apoptotic and nonapoptotic cells.

Key Words: apoptosis; apoptotic cell death; apoptotic marker; quiescence; senescence


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