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Published 26 November 2001. doi:10.1083/jcb.200107008
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© The Rockefeller University Press, 0021-9525/2001/11/797 $5.00
The Journal of Cell Biology, Volume 155, Number 5, November 26, 2001 797-808

Article

A Gip1p–Glc7p phosphatase complex regulates septin organization and spore wall formation



Hiroyuki Tachikawa1, Andrew Bloecher2, Kelly Tatchell2 and Aaron M. Neiman1

1 Department of Biochemistry and Cell Biology, Institute for Cell and Developmental Biology, State University of New York at Stony Brook, Stony Brook, NY 11794
2 Department of Biochemistry and Molecular Biology, Louisiana State University Medical Center, Shreveport, LA 71130

Address correspondence to Aaron Neiman, 332 Life Sciences, Dept. of Biochemistry and Cell Biology, SUNY Stony Brook, Stony Brook, NY 11794-5215. Tel.: (631) 632-1543. Fax: (631) 632-8575. E-mail: Aaron.Neiman{at}sunysb.edu

Sporulation of Saccharomyces cerevisiae is a developmental process in which a single cell is converted into four haploid spores. GIP1, encoding a developmentally regulated protein phosphatase 1 interacting protein, is required for spore formation. Here we show that GIP1 and the protein phosphatase 1 encoded by GLC7 play essential roles in spore development. The gip1{Delta} mutant undergoes meiosis and prospore membrane formation normally, but is specifically defective in spore wall synthesis. We demonstrate that in wild-type cells, distinct layers of the spore wall are deposited in a specific temporal order, and that gip1{Delta} cells display a discrete arrest at the onset of spore wall deposition. Localization studies revealed that Gip1p and Glc7p colocalize with the septins in structures underlying the growing prospore membranes. Interestingly, in the gip1{Delta} mutant, not only is Glc7p localization altered, but septins are also delocalized. Similar phenotypes were observed in a glc7–136 mutant, which expresses a Glc7p defective in interacting with Gip1p. These results indicate that a Gip1p–Glc7p phosphatase complex is required for proper septin organization and initiation of spore wall formation during sporulation.

Key Words: sporulation; GIP1; GLC7; septin; spore wall


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