A role for Vps1p, actin, and the Myo2p motor in peroxisome abundance and inheritance in Saccharomyces cerevisiae

doi:10.1083/jcb.200107028

Published online 3 December 2001. doi:10.1083/jcb.200107028

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© The Rockefeller University Press, 0021-9525/2001/12/979 $5.00
The Journal of Cell Biology, Volume 155, Number 6, December 10, 2001 979-990

Article

A role for Vps1p, actin, and the Myo2p motor in peroxisome abundance and inheritance in Saccharomyces cerevisiae

Dominic Hoepfner¹, Marlene van den Berg², Peter Philippsen¹, Henk F. Tabak² and Ewald H. Hettema²

¹ Lehrstuhl für Angewandte Mikrobiologie, Biozentrum, Universität Basel, CH-4056 Basel, Switzerland
² Department of Biochemistry, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, Netherlands

Address correspondence to Department of Biochemistry, Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, Netherlands. Tel.: (31) 20-566-5127. Fax: (31) 20-691-5519. E-mail: h.f.tabak{at}amc.uva.nl

In vivo time-lapse microscopy reveals that the number of peroxisomesin Saccharomyces cerevisiae cells is fairly constant and thata subset of the organelles are targeted and segregated to thebud in a highly ordered, vectorial process. The dynamin-likeprotein Vps1p controls the number of peroxisomes, since in avps1 ${Delta}$ mutant only one or two giant peroxisomes remain. Analogousto the function of other dynamin-related proteins, Vps1p maybe involved in a membrane fission event that is required forthe regulation of peroxisome abundance. We found that efficientsegregation of peroxisomes from mother to bud is dependent onthe actin cytoskeleton, and active movement of peroxisomes alongactin filaments is driven by the class V myosin motor protein,Myo2p: (a) peroxisomal dynamics always paralleled the polarityof the actin cytoskeleton, (b) double labeling of peroxisomesand actin cables revealed a close association between both,(c) depolymerization of the actin cytoskeleton abolished allperoxisomal movements, and (d) in cells containing thermosensitivealleles of MYO2, all peroxisome movement immediately stoppedat the nonpermissive temperature. In addition, time-lapse videosshowing peroxisome movement in wild-type and vps1 ${Delta}$ cells suggestthe existence of various levels of control involved in the partitioningof peroxisomes.

Key Words: peroxisome inheritance; Vps1; fission; actin cytoskeleton; Myo2p

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