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Liwen Jiang¹, Thomas E. Phillips², Christopher A. Hamm³, Yolanda M. Drozdowicz⁴, Philip A. Rea⁴, Masayoshi Maeshima⁵, Sally W. Rogers³ and John C. Rogers³

¹ Department of Biology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
² Division of Biological Sciences, University of Missouri, Columbia, MO 65211
³ Institute of Biological Chemistry, Washington State University, Pullman, WA 99164
⁴ Plant Science Institute, Department of Biology, University of Pennsylvania, Philadelphia, PA 19104
⁵ Graduate School of Bioagricultural Science, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan

Address correspondence to John C. Rogers, Institute of Biological Chemistry, Washington State University, Pullman WA 99164-6340. Tel.: (509) 335-2773. Fax: (509) 335-7643. E-mail: bcjroger{at}wsu.edu

Storage proteins are deposited into protein storage vacuoles (PSVs) during plant seed development and maturation and stably accumulate to high levels; subsequently, during germination the storage proteins are rapidly degraded to provide nutrients for use by the embryo. Here, we show that a PSV has within it a membrane-bound compartment containing crystals of phytic acid and proteins that are characteristic of a lytic vacuole. This compound organization, a vacuole within a vacuole whereby storage functions are separated from lytic functions, has not been described previously for organelles within the secretory pathway of eukaryotic cells. The partitioning of storage and lytic functions within the same vacuole may reflect the need to keep the functions separate during seed development and maturation and yet provide a ready source of digestive enzymes to initiate degradative processes early in germination.

Key Words: storage protein; prevacuolar compartment; lytic vacuole; multivesicular body

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