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Published online 17 December 2001. doi:10.1083/jcb.200108051
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© The Rockefeller University Press, 0021-9525/2001/12/1109 $5.00
The Journal of Cell Biology, Volume 155, Number 7, December 24, 2001 1109-1116

Report

Aurora-A kinase is required for centrosome maturation in Caenorhabditis elegans



Eva Hannak1,2, Matthew Kirkham1, Anthony A. Hyman1 and Karen Oegema1,2

1 Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
2 European Molecular Biology Laboratory, 69117 Heidelberg, Germany

Address correspondence to Anthony A. Hyman, MPI for Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany. Tel.: 49-351-210-1700. Fax: 49-351-210-1289. E-mail: hyman{at}mpi-cbg.de

Centrosomes mature as cells enter mitosis, accumulating {gamma}-tubulin and other pericentriolar material (PCM) components. This occurs concomitant with an increase in the number of centrosomally organized microtubules (MTs). Here, we use RNA-mediated interference (RNAi) to examine the role of the aurora-A kinase, AIR-1, during centrosome maturation in Caenorhabditis elegans. In air-1(RNAi) embryos, centrosomes separate normally, an event that occurs before maturation in C. elegans. After nuclear envelope breakdown, the separated centrosomes collapse together, and spindle assembly fails. In mitotic air-1(RNAi) embryos, centrosomal {alpha}-tubulin fluorescence intensity accumulates to only 40% of wild-type levels, suggesting a defect in the maturation process. Consistent with this hypothesis, we find that AIR-1 is required for the increase in centrosomal {gamma}-tubulin and two other PCM components, ZYG-9 and CeGrip, as embryos enter mitosis. Furthermore, the AIR-1–dependent increase in centrosomal {gamma}-tubulin does not require MTs. These results suggest that aurora-A kinases are required to execute a MT-independent pathway for the recruitment of PCM during centrosome maturation.

Key Words: microtubule; mitosis; cell cycle; cancer


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