Aurora-A kinase is required for centrosome maturation in Caenorhabditis elegans

doi:10.1083/jcb.200108051

Published online 17 December 2001. doi:10.1083/jcb.200108051

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© The Rockefeller University Press, 0021-9525/2001/12/1109 $5.00
The Journal of Cell Biology, Volume 155, Number 7, December 24, 2001 1109-1116

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Aurora-A kinase is required for centrosome maturation in Caenorhabditis elegans

Eva Hannak¹^,2, Matthew Kirkham¹, Anthony A. Hyman¹ and Karen Oegema¹^,2

¹ Max Planck Institute of Molecular Cell Biology and Genetics, 01307 Dresden, Germany
² European Molecular Biology Laboratory, 69117 Heidelberg, Germany

Address correspondence to Anthony A. Hyman, MPI for Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany. Tel.: 49-351-210-1700. Fax: 49-351-210-1289. E-mail: hyman{at}mpi-cbg.de

Centrosomes mature as cells enter mitosis, accumulating ${gamma}$ -tubulinand other pericentriolar material (PCM) components. This occursconcomitant with an increase in the number of centrosomallyorganized microtubules (MTs). Here, we use RNA-mediated interference(RNAi) to examine the role of the aurora-A kinase, AIR-1, duringcentrosome maturation in Caenorhabditis elegans. In air-1(RNAi)embryos, centrosomes separate normally, an event that occursbefore maturation in C. elegans. After nuclear envelope breakdown,the separated centrosomes collapse together, and spindle assemblyfails. In mitotic air-1(RNAi) embryos, centrosomal ${alpha}$ -tubulinfluorescence intensity accumulates to only 40% of wild-typelevels, suggesting a defect in the maturation process. Consistentwith this hypothesis, we find that AIR-1 is required for theincrease in centrosomal ${gamma}$ -tubulin and two other PCM components,ZYG-9 and CeGrip, as embryos enter mitosis. Furthermore, theAIR-1–dependent increase in centrosomal ${gamma}$ -tubulin doesnot require MTs. These results suggest that aurora-A kinasesare required to execute a MT-independent pathway for the recruitmentof PCM during centrosome maturation.

Key Words: microtubule; mitosis; cell cycle; cancer

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