JCB logo
Accuri Cytometers
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published 7 January 2002. doi:10.1083/jcb.200110086
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 231K)
Right arrow PPT slides of all figures
Right arrow Supplemental Material Index
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schott, D. H.
Right arrow Articles by Bretscher, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schott, D. H.
Right arrow Articles by Bretscher, A.
Right arrowPubmed/NCBI databases
*Protein
*Substance via MeSH
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

© The Rockefeller University Press, 0021-9525/2002/1/35 $5.00
The Journal of Cell Biology, Volume 156, Number 1, January 7, 2002 35-40

Report

Secretory vesicle transport velocity in living cells depends on the myosin-V lever arm length



Daniel H. Schott1, Ruth N. Collins2 and Anthony Bretscher1

1 Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853
2 Department of Molecular Medicine, Cornell University, Ithaca, NY 14853

Address correspondence to Anthony Bretscher, Dept. of Molecular Biology and Genetics, 351 Biotechnology Bldg., Cornell University, Ithaca, NY 14853. Tel.: (607) 255-5713. Fax: (607) 255-2428. E-mail: apb5{at}cornell.edu

Myosins are molecular motors that exert force against actin filaments. One widely conserved myosin class, the myosin-Vs, recruits organelles to polarized sites in animal and fungal cells. However, it has been unclear whether myosin-Vs actively transport organelles, and whether the recently challenged lever arm model developed for muscle myosin applies to myosin-Vs. Here we demonstrate in living, intact yeast that secretory vesicles move rapidly toward their site of exocytosis. The maximal speed varies linearly over a wide range of lever arm lengths genetically engineered into the myosin-V heavy chain encoded by the MYO2 gene. Thus, secretory vesicle polarization is achieved through active transport by a myosin-V, and the motor mechanism is consistent with the lever arm model.

Key Words: exocytosis; Saccharomyces; molecular motors; myosin; cell polarity


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents