{beta}IV-spectrin regulates sodium channel clustering through ankyrin-G at axon initial segments and nodes of Ranvier

doi:10.1083/jcb.200110003

Published 21 January 2002. doi:10.1083/jcb.200110003

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© The Rockefeller University Press, 0021-9525/2002/1/337 $5.00
The Journal of Cell Biology, Volume 156, Number 2, January 21, 2002 337-348

Article

ßIV-spectrin regulates sodium channel clustering through ankyrin-G at axon initial segments and nodes of Ranvier

Masayuki Komada¹^,2 and Philippe Soriano¹

¹ Program in Developmental Biology and Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109
² Department of Biological Sciences, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama 226-8501, Japan

Address correspondence to Masayuki Komada, Dept. of Life Sciences, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan. Tel.: 81-45-924-5702. Fax: 81-45-924-5771. E-mail: makomada{at}bio.titech.ac.jp

ß-Spectrin and ankyrin are major components of themembrane cytoskeleton. We have generated mice carrying a nullmutation in the ßIV-spectrin gene using gene trappingin embryonic stem cells. Mice homozygous for the mutation exhibittremors and contraction of hindlimbs. ßIV-spectrinexpression is mostly restricted to neurons, where it colocalizeswith and binds to ankyrin-G at axon initial segments (AISs)and nodes of Ranvier (NR). In ßIV-spectrin–nullneurons, neither ankyrin-G nor voltage-gated sodium channels(VGSC) are correctly clustered at these sites, suggesting thatimpaired action potential caused by mislocalization of VGSCleads to the phenotype. Conversely, in ankyrin-G–nullneurons, ßIV-spectrin is not localized to these sites.These results indicate that ßIV-spectrin and ankyrin-Gmutually stabilize the membrane protein cluster and the linkedmembrane cytoskeleton at AIS and NR.

Key Words: ßIV-spectrin; ankyrin-G; voltage-gated sodium channel; axon initial segment; node of Ranvier

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