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Published 4 February 2002. doi:10.1083/jcb.200108135
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© The Rockefeller University Press, 0021-9525/2002/2/437 $5.00
The Journal of Cell Biology, Volume 156, Number 3, February 4, 2002 437-451

Article

Drosophila Aurora A kinase is required to localize D-TACC to centrosomes and to regulate astral microtubules



Régis Giet1, Doris McLean2, Simon Descamps3, Michael J. Lee4, Jordan W. Raff4, Claude Prigent3 and David M. Glover1

1 Department of Genetics, Cancer Research Campaign Cell Cycle Genetics Group, University of Cambridge, Cambridge CB2 3EH, UK
2 Department of Surgery and Molecular Oncology, Ninewells Hospital and Medical School, University of Dundee, Dundee DD1 9SY, UK
3 UMR 6061 Génétique et Développement, Centre National de la Recherche Scientifique, Faculté de Médecine, Université de Rennes 1, 35043 Rennes cedex, France
4 Wellcome/CRC Institute and Department of Genetics, Cambridge CB2 1QR, UK

Address correspondence to David M. Glover, Dept. of Genetics, University of Cambridge, Downing St., Cambridge CB2 3EH, United Kingdom. Tel.: 44-1223-333-988. Fax: 44-1223-333-968. E-mail: dmg25{at}mole.bio.cam.ac.uk

Disruption of the function of the A-type Aurora kinase of Drosophila by mutation or RNAi leads to a reduction in the length of astral microtubules in syncytial embryos, larval neuroblasts, and cultured S2 cells. In neuroblasts, it can also lead to loss of an organized centrosome and its associated aster from one of the spindle poles, whereas the centrosome at the other pole has multiple centrioles. When centrosomes are present at the poles of aurA mutants or aurA RNAi spindles, they retain many antigens but are missing the Drosophila counterpart of mammalian transforming acidic coiled coil (TACC) proteins, D-TACC. We show that a subpopulation of the total Aurora A is present in a complex with D-TACC, which is a substrate for the kinase. We propose that one of the functions of Aurora A kinase is to direct centrosomal organization such that D-TACC complexed to the MSPS/XMAP215 microtubule-associated protein may be recruited, and thus modulate the behavior of astral microtubules.

Key Words: Aurora A; D-TACC; mitosis; centrosomes; microtubule


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