Visualization of Rab9-mediated vesicle transport from endosomes to the trans-Golgi in living cells

doi:10.1083/jcb.200109030

Published 4 February 2002. doi:10.1083/jcb.200109030

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© The Rockefeller University Press, 0021-9525/2002/2/511 $5.00
The Journal of Cell Biology, Volume 156, Number 3, February 4, 2002 511-518

Article

Visualization of Rab9-mediated vesicle transport from endosomes to the trans-Golgi in living cells

Pierre Barbero, Lenka Bittova and Suzanne R. Pfeffer

Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305

Address correspondence to Suzanne R. Pfeffer, Dept. of Biochemistry, B400, Stanford University School of Medicine, Stanford, CA 94305-5307. Tel.: (650) 723-6169. Fax: (650) 723-6783. E-mail: pfeffer{at}cmgm.stanford.edu

Mannose 6-phosphate receptors (MPRs) are transported from endosomesto the trans-Golgi via a transport process that requires theRab9 GTPase and the cargo adaptor TIP47. We have generated greenfluorescent protein variants of Rab9 and determined their localizationin cultured cells. Rab9 is localized primarily in late endosomesand is readily distinguished from the trans-Golgi marker galactosyltransferase.Coexpression of fluorescent Rab9 and Rab7 revealed that thesetwo late endosome Rabs occupy distinct domains within late endosomemembranes. Cation-independent mannose 6-phosphate receptorsare enriched in the Rab9 domain relative to the Rab7 domain.TIP47 is likely to be present in this domain because it colocalizeswith the receptors in fixed cells, and a TIP47 mutant disruptedendosome morphology and sequestered MPRs intracellularly. Rab9is present on endosomes that display bidirectional microtubule-dependentmotility. Rab9-positive transport vesicles fuse with the trans-Golginetwork as followed by video microscopy of live cells. Thesedata provide the first indication that Rab9-mediated endosometo trans-Golgi transport can use a vesicle (rather than a tubular)intermediate. Our data suggest that Rab9 remains vesicle associateduntil docking with the Golgi complex and is rapidly removedconcomitant with or just after membrane fusion.

Key Words: endosome; Rab9; Golgi complex; Rab7; TIP47

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