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Published online 11 February 2002. doi:10.1083/jcb.200110081
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© The Rockefeller University Press, 0021-9525/2002/2/653 $5.00
The Journal of Cell Biology, Volume 156, Number 4, February 18, 2002 653-664

Article

 Early/recycling endosomes-to-TGN transport involves two SNARE complexes and a Rab6 isoform

Frédéric Mallard1, Bor Luen Tang3, Thierry Galli1,2, Danièle Tenza1, Agnès Saint-Pol1, Xu Yue3, Claude Antony1, Wanjin Hong3, Bruno Goud1 and Ludger Johannes1

1 UMR144 Curie/CNRS, Institut Curie, F-75248 Paris Cedex 05, France
2 INSERM U536, Institut Curie, F-75248 Paris Cedex 05, France
3 Membrane Biology Laboratory, Institute of Molecular and Cell Biology, Singapore 117609, Republic of Singapore

Address correspondence to Ludger Johannes, Traffic and Signaling Laboratory, UMR144 Institut Curie/CNRS, 26 rue d'Ulm, F-75248 Paris Cedex 05, France. Tel.: 33-1-42346351. Fax: 33-1-42346507. E-mail: johannes{at}curie.fr

The molecular mechanisms underlying early/recycling endosomes-to-TGN transport are still not understood. We identified interactions between the TGN-localized putative t-SNAREs syntaxin 6, syntaxin 16, and Vti1a, and two early/recycling endosomal v-SNAREs, VAMP3/cellubrevin, and VAMP4. Using a novel permeabilized cell system, these proteins were functionally implicated in the post-Golgi retrograde transport step. The function of Rab6a' was also required, whereas its closely related isoform, Rab6a, has previously been implicated in Golgi-to-endoplasmic reticulum transport. Thus, our study shows that membrane exchange between the early endocytic and the biosynthetic/secretory pathways involves specific components of the Rab and SNARE machinery, and suggests that retrograde transport between early/recycling endosomes and the endoplasmic reticulum is critically dependent on the sequential action of two members of the Rab6 subfamily.

Key Words: shiga toxin; early/recycling endosomes; SNARE; Rab protein; retrograde transport


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