JCB logo
Accuri Cytometers
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published online 25 February 2002. doi:10.1083/jcb.200109056
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 337K)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lavoie, B. D.
Right arrow Articles by Koshland, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lavoie, B. D.
Right arrow Articles by Koshland, D.
Right arrowPubmed/NCBI databases
*Gene*GEO Profiles
*HomoloGene
*Substance via MeSH
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

© The Rockefeller University Press, 0021-9525/2002/3/805 $5.00
The Journal of Cell Biology, Volume 156, Number 5, March 4, 2002 805-815

Article

In vivo dissection of the chromosome condensation machinery

: reversibility of condensation distinguishes contributions of condensin and cohesin



Brigitte D. Lavoie1, Eileen Hogan2 and Douglas Koshland2

1 Department of Medical Genetics and Microbiology, University of Toronto, Toronto, ON M5S 1A8, Canada
2 Department of Embryology, Howard Hughes Medical Institute, Carnegie Institution of Washington, Baltimore, MD 21210

Address correspondence to Brigitte D. Lavoie, Department of Medical Genetics and Microbiology, University of Toronto, Medical Sciences Building, Room 4278, Toronto, ON M5S 1A8, Canada. Tel.: (416) 978-6123. Fax: (416) 978-6885. E-mail: brigitte.lavoie{at}utoronto.ca

The machinery mediating chromosome condensation is poorly understood. To begin to dissect the in vivo function(s) of individual components, we monitored mitotic chromosome structure in mutants of condensin, cohesin, histone H3, and topoisomerase II (topo II). In budding yeast, both condensation establishment and maintenance require all of the condensin subunits, but not topo II activity or phospho-histone H3. Structural maintenance of chromosome (SMC) protein 2, as well as each of the three non-SMC proteins (Ycg1p, Ycs4p, and Brn1p), was required for chromatin binding of the condensin complex in vivo. Using reversible condensin alleles, we show that chromosome condensation does not involve an irreversible modification of condensin or chromosomes. Finally, we provide the first evidence of a mechanistic link between condensin and cohesin function. A model discussing the functional interplay between cohesin and condensin is presented.

Key Words: sister chromatid cohesion; mitotic chromosome structure; SMC proteins; chromosome dynamics; MCD1/SCC1


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents