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Published online 11 March 2002. doi:10.1083/jcb.20112068
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© The Rockefeller University Press, 0021-9525/2002/3/1077 $5.00
The Journal of Cell Biology, Volume 156, Number 6, March 18, 2002 1077-1087

Article

The role of ARK in stress-induced apoptosis in Drosophila cells



Katja C. Zimmermann, Jean-Ehrland Ricci, Nathalie M. Droin and Douglas R. Green

Division of Cellular Immunology, La Jolla Institute for Allergy and Immunology, San Diego, California 92121

Address correspondence to Douglas R. Green, La Jolla Institute for Allergy and Immunology, 10355 Science Center Dr., San Diego, CA 92121. Tel.: (858) 558-3500. Fax: (858) 558-3526. E-mail: dgreen5240{at}aol.com

The molecular mechanisms of apoptosis are highly conserved throughout evolution. The homologs of genes essential for apoptosis in Caenorhabditis elegans and Drosophila melanogaster have been shown to be important for apoptosis in mammalian systems. Although a homologue for CED-4/apoptotic protease-activating factor (Apaf)-1 has been described in Drosophila, its exact function and the role of the mitochondrial pathway in its activation remain unclear. Here, we used the technique of RNA interference to dissect apoptotic signaling pathways in Drosophila cells. Inhibition of the Drosophila CED-4/Apaf-1–related killer (ARK) homologue resulted in pronounced inhibition of stress-induced apoptosis, whereas loss of ARK did not protect the cells from Reaper- or Grim-induced cell death. Reduction of DIAP1 induced rapid apoptosis in these cells, whereas the inhibition of DIAP2 expression did not but resulted in increased sensitivity to stress-induced apoptosis; apoptosis in both cases was prevented by inhibition of ARK expression. Cells in which cytochrome c expression was decreased underwent apoptosis induced by stress stimuli, Reaper or Grim. These results demonstrate the central role of ARK in stress-induced apoptosis, which appears to act independently of cytochrome c. Apoptosis induced by Reaper or Grim can proceed via a distinct pathway, independent of ARK.

Key Words: apoptosis; ARK; DIAP; mitochondria; Drosophila


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