Claudin-based tight junctions are crucial for the mammalian epidermal barrier: a lesson from claudin-1-deficient mice

doi:10.1083/jcb.200110122

Published online 11 March 2002. doi:10.1083/jcb.200110122

This Article

	Full Text
	Full Text (PDF, 863K)
	PPT slides of all figures
	Alert me when this article is cited
	Citation Map

Services

	Email this article
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new content in the JCB
	Download to citation manager

Citing Articles

	Citing Articles via HighWire
	Citing Articles via CrossRef
	Citing Articles via Google Scholar

Google Scholar

	Articles by Furuse, M.
	Articles by Tsukita, S.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Furuse, M.
	Articles by Tsukita, S.


Related Collections

	Related Article

Social Bookmarking

	What's this?

© The Rockefeller University Press, 0021-9525/2002/3/1099 $5.00
The Journal of Cell Biology, Volume 156, Number 6, March 18, 2002 1099-1111

Article

Claudin-based tight junctions are crucial for the mammalian epidermal barrier

: a lesson from claudin-1–deficient mice

Mikio Furuse¹, Masaki Hata², Kyoko Furuse², Yoko Yoshida³, Akinori Haratake⁴, Yoshinobu Sugitani⁵, Tetsuo Noda⁵^,6, Akiharu Kubo¹ and Shoichiro Tsukita¹

¹ Department of Cell Biology, Kyoto University Faculty of Medicine, Yoshida-Konoe, Sakyo-ku, Kyoto 606-8501, Japan
² KAN Research Institute, Inc., Kyoto Research Park, Chudoji, Shimogyo-ku, Kyoto 600-8317, Japan
³ Department of Dermatology, Kyoto University Faculty of Medicine, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan
⁴ Basic Research Laboratory, Kanebo, Ltd., Kotobuki-cho, Odawara, Kanagawa 250-0002, Japan
⁵ Department of Cell Biology, Japanese Foundation for Cancer Research-Cancer Institute, Kami-Ikebukuro, Toshima-ku, Tokyo 170-8455, Japan
⁶ Department of Molecular Genetics, Tohoku University School of Medicine, Seryo-cho, Aoba-ku, Sendai 980-8575, Japan

Address correspondence to Shoichiro Tsukita, Dept. of Cell Biology, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan. Tel.: 81-75-753-4372. Fax: 81-75-753-4660. E-mail: htsukita{at}mfour.med.kyoto-u.ac.jp

The tight junction (TJ) and its adhesion molecules, claudins,are responsible for the barrier function of simple epithelia,but TJs have not been thought to play an important role in thebarrier function of mammalian stratified epithelia, includingthe epidermis. Here we generated claudin-1–deficient miceand found that the animals died within 1 d of birth with wrinkledskin. Dehydration assay and transepidermal water loss measurementsrevealed that in these mice the epidermal barrier was severelyaffected, although the layered organization of keratinocytesappeared to be normal. These unexpected findings prompted usto reexamine TJs in the epidermis of wild-type mice. Close inspectionby immunofluorescence microscopy with an antioccludin monoclonalantibody, a TJ-specific marker, identified continuous TJs inthe stratum granulosum, where claudin-1 and -4 were concentrated.The occurrence of TJs was also confirmed by ultrathin sectionEM. In claudin-1–deficient mice, claudin-1 appeared tohave simply been removed from these TJs, leaving occludin-positive(and also claudin-4–positive) TJs. Interestingly, in thewild-type epidermis these occludin-positive TJs efficientlyprevented the diffusion of subcutaneously injected tracer ( $~$ 600D) toward the skin surface, whereas in the claudin-1–deficientepidermis the tracer appeared to pass through these TJs. Thesefindings provide the first evidence that continuous claudin-basedTJs occur in the epidermis and that these TJs are crucial forthe barrier function of the mammalian skin.

Key Words: claudin-1; tight junction; skin; epidermis; barrier

Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Facebook Add to Reddit Reddit Add to Technorati Technorati Twitter What's this?

Related Article

Only one way to skin a mouse: Alan W. Dove
J. Cell Biol. 2002 156: 939. [Full Text] [PDF]

This article has been cited by other articles:

D'Souza, T., Sherman-Baust, C. A., Poosala, S., Mullin, J. M., Morin, P. J. (2009). Age-Related Changes of Claudin Expression in Mouse Liver, Kidney, and Pancreas. J Gerontol A Biol Sci Med Sci 64A: 1146-1153 [Abstract] [Full Text]
List, K., Kosa, P., Szabo, R., Bey, A. L., Wang, C. B., Molinolo, A., Bugge, T. H. (2009). Epithelial Integrity Is Maintained by a Matriptase-Dependent Proteolytic Pathway. Am. J. Pathol. 175: 1453-1463 [Abstract] [Full Text]
Franke, W. W. (2009). Discovering the Molecular Components of Intercellular Junctions--A Historical View. Cold Spring Harb. Perspect. Biol. 1: a003061-a003061 [Abstract] [Full Text]
Kirschner, N., Poetzl, C., von den Driesch, P., Wladykowski, E., Moll, I., Behne, M. J., Brandner, J. M. (2009). Alteration of Tight Junction Proteins Is an Early Event in Psoriasis: Putative Involvement of Proinflammatory Cytokines. Am. J. Pathol. 175: 1095-1106 [Abstract] [Full Text]
Winkler, L., Gehring, C., Wenzel, A., Muller, S. L., Piehl, C., Krause, G., Blasig, I. E., Piontek, J. (2009). Molecular Determinants of the Interaction between Clostridium perfringens Enterotoxin Fragments and Claudin-3. J. Biol. Chem. 284: 18863-18872 [Abstract] [Full Text]
Swystun, V. A., Renaux, B., Moreau, F., Wen, S., Peplowski, M. A., Hollenberg, M. D., MacNaughton, W. K. (2009). Serine proteases decrease intestinal epithelial ion permeability by activation of protein kinase C{zeta}. Am. J. Physiol. Gastrointest. Liver Physiol. 297: G60-G70 [Abstract] [Full Text]
Medigeshi, G. R., Hirsch, A. J., Brien, J. D., Uhrlaub, J. L., Mason, P. W., Wiley, C., Nikolich-Zugich, J., Nelson, J. A. (2009). West Nile Virus Capsid Degradation of Claudin Proteins Disrupts Epithelial Barrier Function. J. Virol. 83: 6125-6134 [Abstract] [Full Text]
Yoshida, Y., Ban, Y., Kinoshita, S. (2009). Tight Junction Transmembrane Protein Claudin Subtype Expression and Distribution in Human Corneal and Conjunctival Epithelium. IOVS 50: 2103-2108 [Abstract] [Full Text]
Epple, H-J, Schneider, T, Troeger, H, Kunkel, D, Allers, K, Moos, V, Amasheh, M, Loddenkemper, C, Fromm, M, Zeitz, M, Schulzke, J-D (2009). Impairment of the intestinal barrier is evident in untreated but absent in suppressively treated HIV-infected patients. Gut 58: 220-227 [Abstract] [Full Text]
Harten, S. K., Shukla, D., Barod, R., Hergovich, A., Balda, M. S., Matter, K., Esteban, M. A., Maxwell, P. H. (2009). Regulation of Renal Epithelial Tight Junctions by the von Hippel-Lindau Tumor Suppressor Gene Involves Occludin and Claudin 1 and Is Independent of E-Cadherin. Mol. Biol. Cell 20: 1089-1101 [Abstract] [Full Text]
Turgeon, B., Meloche, S. (2009). Interpreting Neonatal Lethal Phenotypes in Mouse Mutants: Insights Into Gene Function and Human Diseases. Physiol. Rev. 89: 1-26 [Abstract] [Full Text]
Maria, O. M., Kim, J.-W. M., Gerstenhaber, J. A., Baum, B. J., Tran, S. D. (2008). Distribution of Tight Junction Proteins in Adult Human Salivary Glands. J. Histochem. Cytochem. 56: 1093-1098 [Abstract] [Full Text]
Ferrari, A., Veligodskiy, A., Berge, U., Lucas, M. S., Kroschewski, R. (2008). ROCK-mediated contractility, tight junctions and channels contribute to the conversion of a preapical patch into apical surface during isochoric lumen initiation. J. Cell Sci. 121: 3649-3663 [Abstract] [Full Text]
Fritzsche, F. R., Oelrich, B., Johannsen, M., Kristiansen, I., Moch, H., Jung, K., Kristiansen, G. (2008). Claudin-1 Protein Expression is a Prognostic Marker of Patient Survival in Renal Cell Carcinomas. Clin. Cancer Res. 14: 7035-7042 [Abstract] [Full Text]
Tinkle, C. L., Pasolli, H. A., Stokes, N., Fuchs, E. (2008). New insights into cadherin function in epidermal sheet formation and maintenance of tissue integrity. Proc. Natl. Acad. Sci. USA 105: 15405-15410 [Abstract] [Full Text]
Angelow, S., Ahlstrom, R., Yu, A. S. L. (2008). Biology of claudins. Am. J. Physiol. Renal Physiol. 295: F867-F876 [Abstract] [Full Text]
Lyons, J. G., Patel, V., Roue, N. C., Fok, S. Y., Soon, L. L., Halliday, G. M., Gutkind, J. S. (2008). Snail Up-regulates Proinflammatory Mediators and Inhibits Differentiation in Oral Keratinocytes. Cancer Res. 68: 4525-4530 [Abstract] [Full Text]
Amasheh, M., Schlichter, S., Amasheh, S., Mankertz, J., Zeitz, M., Fromm, M., Schulzke, J. D. (2008). Quercetin Enhances Epithelial Barrier Function and Increases Claudin-4 Expression in Caco-2 Cells. J. Nutr. 138: 1067-1073 [Abstract] [Full Text]
Charles, R.-P., Guitard, M., Leyvraz, C., Breiden, B., Haftek, M., Haftek-Terreau, Z., Stehle, J.-C., Sandhoff, K., Hummler, E. (2008). Postnatal Requirement of the Epithelial Sodium Channel for Maintenance of Epidermal Barrier Function. J. Biol. Chem. 283: 2622-2630 [Abstract] [Full Text]
Fontijn, R. D., Volger, O. L., Fledderus, J. O., Reijerkerk, A., de Vries, H. E., Horrevoets, A. J. G. (2008). SOX-18 controls endothelial-specific claudin-5 gene expression and barrier function. Am. J. Physiol. Heart Circ. Physiol. 294: H891-H900 [Abstract] [Full Text]
Yamamoto, M., Ramirez, S. H., Sato, S., Kiyota, T., Cerny, R. L., Kaibuchi, K., Persidsky, Y., Ikezu, T. (2008). Phosphorylation of Claudin-5 and Occludin by Rho Kinase in Brain Endothelial Cells. Am. J. Pathol. 172: 521-533 [Abstract] [Full Text]
Piontek, J., Winkler, L., Wolburg, H., Muller, S. L., Zuleger, N., Piehl, C., Wiesner, B., Krause, G., Blasig, I. E. (2008). Formation of tight junction: determinants of homophilic interaction between classic claudins. FASEB J. 22: 146-158 [Abstract] [Full Text]
Yano, K., Imaeda, T., Niimi, T. (2008). Transcriptional activation of the human claudin-18 gene promoter through two AP-1 motifs in PMA-stimulated MKN45 gastric cancer cells. Am. J. Physiol. Gastrointest. Liver Physiol. 294: G336-G343 [Abstract] [Full Text]
Demerjian, M., Hachem, J.-P., Tschachler, E., Denecker, G., Declercq, W., Vandenabeele, P., Mauro, T., Hupe, M., Crumrine, D., Roelandt, T., Houben, E., Elias, P. M., Feingold, K. R. (2008). Acute Modulations in Permeability Barrier Function Regulate Epidermal Cornification: Role of Caspase-14 and the Protease-Activated Receptor Type 2. Am. J. Pathol. 172: 86-97 [Abstract] [Full Text]
Boudreau, F., Lussier, C. R., Mongrain, S., Darsigny, M., Drouin, J. L., Doyon, G., Suh, E. R., Beaulieu, J.-F., Rivard, N., Perreault, N. (2007). Loss of cathepsin L activity promotes claudin-1 overexpression and intestinal neoplasia. FASEB J. 21: 3853-3865 [Abstract] [Full Text]
Fadloun, A., Kobi, D., Pointud, J.-C., Indra, A. K., Teletin, M., Bole-Feysot, C., Testoni, B., Mantovani, R., Metzger, D., Mengus, G., Davidson, I. (2007). The TFIID subunit TAF4 regulates keratinocyte proliferation and has cell-autonomous and non-cell-autonomous tumour suppressor activity in mouse epidermis. Development 134: 2947-2958 [Abstract] [Full Text]
Trzpis, M., McLaughlin, P. M.J., de Leij, L. M.F.H., Harmsen, M. C. (2007). Epithelial Cell Adhesion Molecule: More than a Carcinoma Marker and Adhesion Molecule. Am. J. Pathol. 171: 386-395 [Abstract] [Full Text]
Wakamatsu, K., Ogita, H., Okabe, N., Irie, K., Tanaka-Okamoto, M., Ishizaki, H., Ishida-Yamamoto, A., Iizuka, H., Miyoshi, J., Takai, Y. (2007). Up-regulation of Loricrin Expression by Cell Adhesion Molecule Nectin-1 through Rap1-ERK Signaling in Keratinocytes. J. Biol. Chem. 282: 18173-18181 [Abstract] [Full Text]
Madan, P., Rose, K., Watson, A. J. (2007). Na/K-ATPase beta1 Subunit Expression Is Required for Blastocyst Formation and Normal Assembly of Trophectoderm Tight Junction-associated Proteins. J. Biol. Chem. 282: 12127-12134 [Abstract] [Full Text]
Epp, N., Furstenberger, G., Muller, K., de Juanes, S., Leitges, M., Hausser, I., Thieme, F., Liebisch, G., Schmitz, G., Krieg, P. (2007). 12R-lipoxygenase deficiency disrupts epidermal barrier function. JCB 177: 173-182 [Abstract] [Full Text]
Troeger, H., Epple, H.-J., Schneider, T., Wahnschaffe, U., Ullrich, R., Burchard, G.-D., Jelinek, T., Zeitz, M., Fromm, M., Schulzke, J.-D. (2007). Effect of chronic Giardia lamblia infection on epithelial transport and barrier function in human duodenum. Gut 56: 328-335 [Abstract] [Full Text]
Timmons, B. C., Mitchell, S. M., Gilpin, C., Mahendroo, M. S. (2007). Dynamic Changes in the Cervical Epithelial Tight Junction Complex and Differentiation Occur during Cervical Ripening and Parturition. Endocrinology 148: 1278-1287 [Abstract] [Full Text]
Sosnova-Netukova, M., Kuchynka, P., Forrester, J. V (2007). The suprabasal layer of corneal epithelial cells represents the major barrier site to the passive movement of small molecules and trafficking leukocytes. Br J Ophthalmol 91: 372-378 [Abstract] [Full Text]
Kawedia, J. D., Nieman, M. L., Boivin, G. P., Melvin, J. E., Kikuchi, K.-I., Hand, A. R., Lorenz, J. N., Menon, A. G. (2007). Interaction between transcellular and paracellular water transport pathways through Aquaporin 5 and the tight junction complex. Proc. Natl. Acad. Sci. USA 104: 3621-3626 [Abstract] [Full Text]
Straight, S. W., Pieczynski, J. N., Whiteman, E. L., Liu, C.-J., Margolis, B. (2006). Mammalian Lin-7 Stabilizes Polarity Protein Complexes. J. Biol. Chem. 281: 37738-37747 [Abstract] [Full Text]
Turner, J. R. (2006). Molecular Basis of Epithelial Barrier Regulation: From Basic Mechanisms to Clinical Application. Am. J. Pathol. 169: 1901-1909 [Abstract] [Full Text]
Geng, S., Mezentsev, A., Kalachikov, S., Raith, K., Roop, D. R., Panteleyev, A. A. (2006). Targeted ablation of Arnt in mouse epidermis results in profound defects in desquamation and epidermal barrier function. J. Cell Sci. 119: 4901-4912 [Abstract] [Full Text]
de Guzman Strong, C., Wertz, P. W., Wang, C., Yang, F., Meltzer, P. S., Andl, T., Millar, S. E., Ho, I-C., Pai, S.-Y., Segre, J. A. (2006). Lipid defect underlies selective skin barrier impairment of an epidermal-specific deletion of Gata-3. JCB 175: 661-670 [Abstract] [Full Text]
Guttman, J. A., Samji, F. N., Li, Y., Vogl, A. W., Finlay, B. B. (2006). Evidence that Tight Junctions Are Disrupted Due to Intimate Bacterial Contact and Not Inflammation during Attaching and Effacing Pathogen Infection In Vivo. Infect. Immun. 74: 6075-6084 [Abstract] [Full Text]
Matsui, T., Kinoshita-Ida, Y., Hayashi-Kisumi, F., Hata, M., Matsubara, K., Chiba, M., Katahira-Tayama, S., Morita, K., Miyachi, Y., Tsukita, S. (2006). Mouse Homologue of Skin-specific Retroviral-like Aspartic Protease Involved in Wrinkle Formation. J. Biol. Chem. 281: 27512-27525 [Abstract] [Full Text]
Arabzadeh, A., Troy, T.-C., Turksen, K. (2006). Role of the Cldn6 Cytoplasmic Tail Domain in Membrane Targeting and Epidermal Differentiation In Vivo. Mol. Cell. Biol. 26: 5876-5887 [Abstract] [Full Text]
Fujita, H., Chiba, H., Yokozaki, H., Sakai, N., Sugimoto, K., Wada, T., Kojima, T., Yamashita, T., Sawada, N. (2006). Differential Expression and Subcellular Localization of Claudin-7, -8, -12, -13, and -15 Along the Mouse Intestine. J. Histochem. Cytochem. 54: 933-944 [Abstract] [Full Text]
Balkovetz, D. F. (2006). Claudins at the gate: determinants of renal epithelial tight junction paracellular permeability. Am. J. Physiol. Renal Physiol. 290: F572-F579 [Abstract] [Full Text]
Takeichi, M. (2006). Shoichiro Tsukita: a life exploring the molecular architecture of the tight junction. JCB 172: 321-323 [Abstract] [Full Text]
Lillywhite, H. B. (2006). Water relations of tetrapod integument. J. Exp. Biol. 209: 202-226 [Abstract] [Full Text]
King, K.E., Ponnamperuma, R.M., Gerdes, M.J., Tokino, T., Yamashita, T., Baker, C.C., Weinberg, W.C. (2006). Unique domain functions of p63 isotypes that differentially regulate distinct aspects of epidermal homeostasis. Carcinogenesis 27: 53-63 [Abstract] [Full Text]
Haorah, J., Knipe, B., Leibhart, J., Ghorpade, A., Persidsky, Y. (2005). Alcohol-induced oxidative stress in brain endothelial cells causes blood-brain barrier dysfunction. J. Leukoc. Biol. 78: 1223-1232 [Abstract] [Full Text]
Howe, K. L., Reardon, C., Wang, A., Nazli, A., McKay, D. M. (2005). Transforming Growth Factor-{beta} Regulation of Epithelial Tight Junction Proteins Enhances Barrier Function and Blocks Enterohemorrhagic Escherichia coli O157:H7-Induced Increased Permeability. Am. J. Pathol. 167: 1587-1597 [Abstract] [Full Text]
Le Moellic, C., Boulkroun, S., Gonzalez-Nunez, D., Dublineau, I., Cluzeaud, F., Fay, M., Blot-Chabaud, M., Farman, N. (2005). Aldosterone and tight junctions: modulation of claudin-4 phosphorylation in renal collecting duct cells. Am. J. Physiol. Cell Physiol. 289: C1513-C1521 [Abstract] [Full Text]
Meng, J., Holdcraft, R. W., Shima, J. E., Griswold, M. D., Braun, R. E. (2005). Androgens regulate the permeability of the blood-testis barrier. Proc. Natl. Acad. Sci. USA 102: 16696-16700 [Abstract] [Full Text]
Indra, A. K., Dupe, V., Bornert, J.-M., Messaddeq, N., Yaniv, M., Mark, M., Chambon, P., Metzger, D. (2005). Temporally controlled targeted somatic mutagenesis in embryonic surface ectoderm and fetal epidermal keratinocytes unveils two distinct developmental functions of BRG1 in limb morphogenesis and skin barrier formation. Development 132: 4533-4544 [Abstract] [Full Text]
Mo, L., Cheng, J., Lee, E. Y.-H. P., Sun, T.-T., Wu, X.-R. (2005). Gene deletion in urothelium by specific expression of Cre recombinase. Am. J. Physiol. Renal Physiol. 289: F562-F568 [Abstract] [Full Text]
Leyvraz, C., Charles, R.-P., Rubera, I., Guitard, M., Rotman, S., Breiden, B., Sandhoff, K., Hummler, E. (2005). The epidermal barrier function is dependent on the serine protease CAP1/Prss8. JCB 170: 487-496 [Abstract] [Full Text]
Masuyama, A., Kondoh, M., Seguchi, H., Takahashi, A., Harada, M., Fujii, M., Mizuguchi, H., Horiguchi, Y., Watanabe, Y. (2005). Role of N-Terminal Amino Acids in the Absorption-Enhancing Effects of the C-Terminal Fragment of Clostridium perfringens Enterotoxin. J. Pharmacol. Exp. Ther. 314: 789-795 [Abstract] [Full Text]
Alexandre, M. D., Lu, Q., Chen, Y.-H. (2005). Overexpression of claudin-7 decreases the paracellular Cl- conductance and increases the paracellular Na+ conductance in LLC-PK1 cells. J. Cell Sci. 118: 2683-2693 [Abstract] [Full Text]
Takano, K.-i., Kojima, T., Go, M., Murata, M., Ichimiya, S., Himi, T., Sawada, N. (2005). HLA-DR- and CD11c-positive Dendritic Cells Penetrate beyond Well-developed Epithelial Tight Junctions in Human Nasal Mucosa of Allergic Rhinitis. J. Histochem. Cytochem. 53: 611-619 [Abstract] [Full Text]
Kondoh, M., Masuyama, A., Takahashi, A., Asano, N., Mizuguchi, H., Koizumi, N., Fujii, M., Hayakawa, T., Horiguchi, Y., Watanbe, Y. (2005). A Novel Strategy for the Enhancement of Drug Absorption Using a Claudin Modulator. Mol. Pharmacol. 67: 749-756 [Abstract] [Full Text]
Lipschutz, J. H., Li, S., Arisco, A., Balkovetz, D. F. (2005). Extracellular Signal-regulated Kinases 1/2 Control Claudin-2 Expression in Madin-Darby Canine Kidney Strain I and II Cells. J. Biol. Chem. 280: 3780-3788 [Abstract] [Full Text]
Graham, C., Simmons, N. L. (2005). Functional organization of the bovine rumen epithelium. Am. J. Physiol. Regul. Integr. Comp. Physiol. 288: R173-R181 [Abstract] [Full Text]
Go, M., Kojima, T., Takano, K.-i., Murata, M., Ichimiya, S., Tsubota, H., Himi, T., Sawada, N. (2004). Expression and Function of Tight Junctions in the Crypt Epithelium of Human Palatine Tonsils. J. Histochem. Cytochem. 52: 1627-1638 [Abstract] [Full Text]
McLaughlin, J., Padfield, P. J., Burt, J. P. H., O'Neill, C. A. (2004). Ochratoxin A increases permeability through tight junctions by removal of specific claudin isoforms. Am. J. Physiol. Cell Physiol. 287: C1412-C1417 [Abstract] [Full Text]
Guillemot, L., Hammar, E., Kaister, C., Ritz, J., Caille, D., Jond, L., Bauer, C., Meda, P., Citi, S. (2004). Disruption of the cingulin gene does not prevent tight junction formation but alters gene expression. J. Cell Sci. 117: 5245-5256 [Abstract] [Full Text]
Kitajiri, S.-i., Miyamoto, T., Mineharu, A., Sonoda, N., Furuse, K., Hata, M., Sasaki, H., Mori, Y., Kubota, T., Ito, J., Furuse, M., Tsukita, S. (2004). Compartmentalization established by claudin-11-based tight junctions in stria vascularis is required for hearing through generation of endocochlear potential. J. Cell Sci. 117: 5087-5096 [Abstract] [Full Text]
Cereijido, M., Contreras, R. G., Shoshani, L. (2004). Cell Adhesion, Polarity, and Epithelia in the Dawn of Metazoans. Physiol. Rev. 84: 1229-1262 [Abstract] [Full Text]
Lee, M. S., Hanspers, K., Barker, C. S., Korn, A. P., McCune, J. M. (2004). Gene expression profiles during human CD4+ T cell differentiation. Int Immunol 16: 1109-1124 [Abstract] [Full Text]
Acharya, P., Beckel, J., Ruiz, W. G., Wang, E., Rojas, R., Birder, L., Apodaca, G. (2004). Distribution of the tight junction proteins ZO-1, occludin, and claudin-4, -8, and -12 in bladder epithelium. Am. J. Physiol. Renal Physiol. 287: F305-F318 [Abstract] [Full Text]
Bazzoni, G., Dejana, E. (2004). Endothelial Cell-to-Cell Junctions: Molecular Organization and Role in Vascular Homeostasis. Physiol. Rev. 84: 869-901 [Abstract] [Full Text]
Loh, Y. H., Christoffels, A., Brenner, S., Hunziker, W., Venkatesh, B. (2004). Extensive Expansion of the Claudin Gene Family in the Teleost Fish, Fugu rubripes. Genome Res 14: 1248-1257 [Abstract] [Full Text]
Schneeberger, E. E., Lynch, R. D. (2004). The tight junction: a multifunctional complex. Am. J. Physiol. Cell Physiol. 286: C1213-C1228 [Abstract] [Full Text]
Turksen, K., Troy, T.-C. (2004). Barriers built on claudins. J. Cell Sci. 117: 2435-2447 [Abstract] [Full Text]
Bojarski, C., Weiske, J., Schoneberg, T., Schroder, W., Mankertz, J., Schulzke, J.-D., Florian, P., Fromm, M., Tauber, R., Huber, O. (2004). The specific fates of tight junction proteins in apoptotic epithelial cells. J. Cell Sci. 117: 2097-2107 [Abstract] [Full Text]
Guitard, M., Leyvraz, C., Hummler, E. (2004). A Nonconventional Look at Ionic Fluxes in the Skin: Lessons From Genetically Modified Mice. Physiology 19: 75-79 [Abstract] [Full Text]
Singh, A. B., Harris, R. C. (2004). Epidermal Growth Factor Receptor Activation Differentially Regulates Claudin Expression and Enhances Transepithelial Resistance in Madin-Darby Canine Kidney Cells. J. Biol. Chem. 279: 3543-3552 [Abstract] [Full Text]
Wu, V. M., Schulte, J., Hirschi, A., Tepass, U., Beitel, G. J. (2004). Sinuous is a Drosophila claudin required for septate junction organization and epithelial tube size control. JCB 164: 313-323 [Abstract] [Full Text]
Van Itallie, C. M., Anderson, J. M. (2004). The Role of Claudins in Determining Paracellular Charge Selectivity. Proc Am Thorac Soc 1: 38-41 [Abstract] [Full Text]
Wang, X., Matsumoto, H., Zhao, X., Das, S. K., Paria, B. C. (2004). Embryonic signals direct the formation of tight junctional permeability barrier in the decidualizing stroma during embryo implantation. J. Cell Sci. 117: 53-62 [Abstract] [Full Text]
Quan, C., Lu, S.-J. (2003). Identification of genes preferentially expressed in mammary epithelial cells of Copenhagen rat using subtractive hybridization and microarrays. Carcinogenesis 24: 1593-1599 [Abstract] [Full Text]
Rajasekaran, A. K., Rajasekaran, S. A. (2003). Role of Na-K-ATPase in the assembly of tight junctions. Am. J. Physiol. Renal Physiol. 285: F388-F396 [Abstract] [Full Text]
Ben-Yosef, T., Belyantseva, I. A., Saunders, T. L., Hughes, E. D., Kawamoto, K., Van Itallie, C. M., Beyer, L. A., Halsey, K., Gardner, D. J., Wilcox, E. R., Rasmussen, J., Anderson, J. M., Dolan, D. F., Forge, A., Raphael, Y., Camper, S. A., Friedman, T. B. (2003). Claudin 14 knockout mice, a model for autosomal recessive deafness DFNB29, are deaf due to cochlear hair cell degeneration. Hum Mol Genet 12: 2049-2061 [Abstract] [Full Text]
Yoo, J., Nichols, A., Mammen, J., Calvo, I., Song, J. C., Worrell, R. T., Matlin, K., Matthews, J. B. (2003). Bryostatin-1 enhances barrier function in T84 epithelia through PKC-dependent regulation of tight junction proteins. Am. J. Physiol. Cell Physiol. 285: C300-C309 [Abstract] [Full Text]
Herrmann, T., van der Hoeven, F., Grone, H.-J., Stewart, A. F., Langbein, L., Kaiser, I., Liebisch, G., Gosch, I., Buchkremer, F., Drobnik, W., Schmitz, G., Stremmel, W. (2003). Mice with targeted disruption of the fatty acid transport protein 4 (Fatp 4, Slc27a4) gene show features of lethal restrictive dermopathy. JCB 161: 1105-1115 [Abstract] [Full Text]
Jaubert, J., Cheng, J., Segre, J. A. (2003). Ectopic expression of Kruppel like factor 4 (Klf4) accelerates formation of the epidermal permeability barrier. Development 130: 2767-2777 [Abstract] [Full Text]
Matter, K., Balda, M. S. (2003). Holey barrier: claudins and the regulation of brain endothelial permeability. JCB 161: 459-460 [Abstract] [Full Text]
Nitta, T., Hata, M., Gotoh, S., Seo, Y., Sasaki, H., Hashimoto, N., Furuse, M., Tsukita, S. (2003). Size-selective loosening of the blood-brain barrier in claudin-5-deficient mice. JCB 161: 653-660 [Abstract] [Full Text]
Yu, A. S. L., Enck, A. H., Lencer, W. I., Schneeberger, E. E. (2003). Claudin-8 Expression in Madin-Darby Canine Kidney Cells Augments the Paracellular Barrier to Cation Permeation. J. Biol. Chem. 278: 17350-17359 [Abstract] [Full Text]
Seagroves, T. N., Hadsell, D., McManaman, J., Palmer, C., Liao, D., McNulty, W., Welm, B., Wagner, K.-U., Neville, M., Johnson, R. S. (2003). HIF1{alpha} is a critical regulator of secretory differentiation and activation, but not vascular expansion, in the mouse mammary gland. Development 130: 1713-1724 [Abstract] [Full Text]
Amasheh, S., Meiri, N., Gitter, A. H., Schoneberg, T., Mankertz, J., Schulzke, J. D., Fromm, M. (2003). Claudin-2 expression induces cation-selective channels in tight junctions of epithelial cells. J. Cell Sci. 115: 4969-4976 [Abstract] [Full Text]
Knust, E., Bossinger, O. (2002). Composition and Formation of Intercellular Junctions in Epithelial Cells. Science 298: 1955-1959 [Abstract] [Full Text]
Doering, T., Brade, H., Sandhoff, K. (2002). Sphingolipid metabolism during epidermal barrier development in mice. J. Lipid Res. 43: 1727-1733 [Abstract] [Full Text]
Cheng, C. Y., Mruk, D. D. (2002). Cell Junction Dynamics in the Testis: Sertoli-Germ Cell Interactions and Male Contraceptive Development. Physiol. Rev. 82: 825-874 [Abstract] [Full Text]
Wells, W. A. (2002). The youth revolution: cell biology in Japan. JCB 158: 609-614 [Full Text]
Bazzoni, G., Dejana, E. (2002). Keratinocyte junctions and the epidermal barrier: how to make a skin-tight dress. JCB 156: 947-949 [Abstract] [Full Text]
Ghassemifar, M. R., Sheth, B., Papenbrock, T., Leese, H. J., Houghton, F. D., Fleming, T. P. (2002). Occludin TM4-: an isoform of the tight junction protein present in primates lacking the fourth transmembrane domain. J. Cell Sci. 115: 3171-3180 [Abstract] [Full Text]