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Published 13 May 2002. doi:10.1083/jcb.200202052
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© The Rockefeller University Press, 0021-9525/2002/5/603 $5.00
The Journal of Cell Biology, Volume 157, Number 4, May 13, 2002 603-613

Article

Meiotic spindle stability depends on MAPK-interacting and spindle-stabilizing protein (MISS), a new MAPK substrate



Christophe Lefebvrea,b, M. Emilie Terreta,b, Alexandre Djianea,b, Pascale Rassiniera,b, Bernard Maroa,b and Marie-Hélène Verlhaca,b

a Biologie Cellulaire et Moléculaire du Developpement, Centre National de la Recherche Scientifique, Université Pierre et Marie Curie
b 75252 Paris, cedex 05, France

Address correspondence to Marie-Hélène Verlhac, Biologie Cellulaire et Moleculaire du Developpement, UMR 7622, Centre National de la Recherche Scientifique/Université Pierre et Marie Curie, 9 quai Saint Bernard-Bat. C-5, 75252 Paris, cedex 05, France. Tel.: 33-14-427-3401. Fax: 33-14-427-3498. E-mail: marie-helene.verlhac{at}snv.jussieu.fr

Vertebrate oocytes arrest in the second metaphase of meiosis (metaphase II [MII]) by an activity called cytostatic factor (CSF), with aligned chromosomes and stable spindles. Segregation of chromosomes occurs after fertilization. The Mos/.../MAPK (mitogen-activated protein kinases) pathway mediates this MII arrest. Using a two-hybrid screen, we identified a new MAPK partner from a mouse oocyte cDNA library. This protein is unstable during the first meiotic division and accumulates only in MII, where it localizes to the spindle. It is a substrate of the Mos/.../MAPK pathway. The depletion of endogenous RNA coding for this protein by three different means (antisense RNA, double-stranded [ds] RNA, or morpholino oligonucleotides) induces severe spindle defects specific to MII oocytes. Overexpressing the protein from an RNA not targeted by the morpholino rescues spindle destabilization. However, dsRNA has no effect on the first two mitotic divisions. We therefore have discovered a new MAPK substrate involved in maintaining spindle integrity during the CSF arrest of mouse oocytes, called MISS (for MAP kinase–interacting and spindle-stabilizing protein).

Key Words: MISS; MAP kinase; spindle stability; morpholino; mouse


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