Fibroblast growth factor-specific modulation of cellular response by syndecan-4

doi:10.1083/jcb.200112145

Published 13 May 2002. doi:10.1083/jcb.200112145

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© The Rockefeller University Press, 0021-9525/2002/5/715 $5.00
The Journal of Cell Biology, Volume 157, Number 4, May 13, 2002 715-725

Article

Fibroblast growth factor–specific modulation of cellular response by syndecan-4

Arie Horowitz, Eugene Tkachenko and Michael Simons

Angiogenesis Research Center and Section of Cardiology, Department of Medicine, Dartmouth Medical School, Lebanon, NH 03756

Address correspondence to Arie Horowitz, Angiogenesis Research Center and Section of Cardiology, HB-7504, Dept. of Medicine, Dartmouth Medical School, One Medical Center Dr., Lebanon, NH 03756. Tel.: (603) 650-2635. Fax: (603) 653-0510. E-mail: arie.horowitz{at}dartmouth.edu

Proteoglycans participate in growth factor interaction withthe cell surface through their heparan sulfate chains (HS),but it is not known if they are otherwise involved in growthfactor signaling. It appears now that the syndecan-4 core protein,a transmembrane proteoglycan shown previously to bind phosphatidylinositol4,5-bisphosphate (PIP₂) and activate PKC ${alpha}$ , participates in mediatingthe effects of fibroblast growth factor (FGF)2 on cell function.Mutations in the cytoplasmic tail of syndecan-4 that eitherreduced its affinity to PIP₂ (PIP₂^-) or disrupted its postsynapticdensity 95, disk large, zona occludens-1 (PDZ)-dependent binding(PDZ^-) produced a FGF2-specific dominant negative phenotypein endothelial cells as evidenced by the marked decline of theirmigration and proliferation rates and the impairment of theircapacity to form tubes. In both cases, the molecular mechanismwas determined to consist of a decrease in the syndecan-4–dependentactivation of PKC ${alpha}$ . This decrease was caused either by inhibitionof FGF2-induced syndecan-4 dephosphorylation in the case ofthe PDZ^- mutation or by disruption of basolateral targetingof syndecan-4 and its associated PDZ-dependent complex in thecase of the PIP₂^- mutation. These results suggest that PKC ${alpha}$ activationand PDZ-mediated formation of a serine/threonine phosphatase-containingcomplex by syndecan-4 are downstream events of FGF2 signaling.

Key Words: FGF; PDZ; signal transduction; syndecan-4; PKC

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