JCB logo
R&D Systems
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published 28 May 2002. doi:10.1083/jcb.200203034
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 915K)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chevrier, V.
Right arrow Articles by Job, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chevrier, V.
Right arrow Articles by Job, D.
Right arrowPubmed/NCBI databases
*Gene*GEO Profiles
*HomoloGene*Nucleotide
*Protein*UniGene
*Substance via MeSH
Related Collections
Right arrowRelated Article
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
© The Rockefeller University Press, 0021-9525/2002/5/807 $5.00
The Journal of Cell Biology, Volume 157, Number 5, May 28, 2002 807-817

Article

 The Rho-associated protein kinase p160ROCK is required for centrosome positioning

Véronique Chevrier1, Matthieu Piel2, Nora Collomb1, Yasmina Saoudi1, Ronald Frank3, Michel Paintrand1, Shuh Narumiya4, Michel Bornens2 and Didier Job1

1 Institut National de la Santé et de la Recherche Medicale U366, Département de Biologie Moléculaire et Structurale/Cytosqulette, Commissariat à l'Energie Atomique, de Grenoble, 38054 Grenoble Cedex 9, France
2 Institut Curie, Section Recherche, UMR 144 du Centre National de la Recherche Scientifique, 75248 Paris Cedex 05, France
3 AG Molecular Recognition, Gesellschaft für Biotechnologische Forschung, D-38124 Braunschweig, Germany
4 Department of Pharmacology, Kyoto University Faculty of Medicine, Sako-ku, Kyoto 606, Japan

Address correspondence to Didier Job, INSERM U366, DBMS/CS, CEA de Grenoble, 17 Rue des Martyrs, 38054 Grenoble Cedex 9, France. Tel.: 33-04-38-78-51-00. Fax: 33-04-38-78-50-57. E-mail: djob{at}cea.fr

The p160–Rho-associated coiled-coil–containing protein kinase (ROCK) is identified as a new centrosomal component. Using immunofluorescence with a variety of p160ROCK antibodies, immuno EM, and depletion with RNA interference, p160ROCK is principally bound to the mother centriole (MC) and an intercentriolar linker. Inhibition of p160ROCK provoked centrosome splitting in G1 with the MC, which is normally positioned at the cell center and shows little motion during G1, displaying wide excursions around the cell periphery, similar to its migration toward the midbody during cytokinesis. p160ROCK inhibition late after anaphase in mitosis triggered MC migration to the midbody followed by completion of cell division. Thus, p160ROCK is required for centrosome positioning and centrosome-dependent exit from mitosis.

Key Words: centrosome; structure; motion; p160ROCK; cytokinesis


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?

Related Article

ROCK-steady centriole migration
Nicole LeBrasseur
J. Cell Biol. 2002 157: 735. [Full Text] [PDF]



This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents