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Published online 3 June 2002. doi:10.1083/jcb.200201060
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© The Rockefeller University Press, 0021-9525/2002/6/953 $5.00
The Journal of Cell Biology, Volume 157, Number 6, June 10, 2002 953-962

Article

Characterization of Tbc2, a nucleus-encoded factor specifically required for translation of the chloroplast psbC mRNA in Chlamydomonas reinhardtii



Andrea H. Auchincloss1, William Zerges1, Karl Perron1, Jacqueline Girard-Bascou2 and Jean-David Rochaix1

1 Department of Molecular Biology and Department of Plant Biology, University of Geneva, 1211 Geneva 4, Switzerland
2 Institut de Biologie Physico-Chimique, F-75005 Paris, France

Address correspondence to Jean-David Rochaix, Department of Molecular Biology, University of Geneva, 30, Quai Ernest Ansermet, Geneva 1211, Switzerland. Tel.: 41-22-702-6187. Fax: 41-22-702-6868. E-mail: Jean-David.Rochaix{at}molbio.unige.ch

Genetic analysis has revealed that the three nucleus-encoded factors Tbc1, Tbc2, and Tbc3 are involved in the translation of the chloroplast psbC mRNA of the eukaryotic green alga Chlamydomonas reinhardtii. In this study we report the isolation and phenotypic characterization of two new tbc2 mutant alleles and their use for cloning and characterizing the Tbc2 gene by genomic complementation. TBC2 encodes a protein of 1,115 residues containing nine copies of a novel degenerate 38–40 amino acid repeat with a quasiconserved PPPEW motif near its COOH-terminal end. The middle part of the Tbc2 protein displays partial amino acid sequence identity with Crp1, a protein from Zea mays that is implicated in the processing and translation of the chloroplast petA and petD RNAs. The Tbc2 protein is enriched in chloroplast stromal subfractions and is associated with a 400-kD protein complex that appears to play a role in the translation of specifically the psbC mRNA.

Key Words: chloroplast; translation; Chlamydomonas; photosynthetic mutant; protein complex


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