Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

This Article Full Text Full Text (PDF, 756K) PPT slides of all figures Supplemental Material Index Alert me when this article is cited Citation Map Services Email this article Similar articles in this journal Similar articles in PubMed Alert me to new content in the JCB Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Segura-Totten, M. Articles by Wilson, K. L. Search for Related Content PubMed PubMed Citation Articles by Segura-Totten, M. Articles by Wilson, K. L. Pubmed/NCBI databases Gene GEO Profiles HomoloGene Protein UniGene Related Collections Related Article Social Bookmarking                      What's this?

Miriam Segura-Totten¹, Amy K. Kowalski¹, Robert Craigie² and Katherine L. Wilson¹

¹ Department of Cell Biology, The Johns Hopkins University School of Medicine, Baltimore, MD 21205
² Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892

Address correspondence to Katherine L. Wilson, Dept. of Cell Biology, The Johns Hopkins University School of Medicine, 725 N. Wolfe St., Baltimore, MD 21205. Tel.: (410) 955-1801. Fax: (410) 955-4129. E-mail: klwilson{at}jhmi.edu

Barrier-to-autointegration factor (BAF) is a DNA-bridging protein, highly conserved in metazoans. BAF binds directly to LEM (LAP2, emerin, MAN1) domain nuclear membrane proteins, including LAP2 and emerin. We used site-directed mutagenesis and biochemical analysis to map functionally important residues in human BAF, including those required for direct binding to DNA or emerin. We also tested wild-type BAF and 25 point mutants for their effects on nuclear assembly in Xenopus egg extracts, which contain 12 µM endogenous BAF dimers. Exogenous BAF caused two distinct effects: at low added concentrations, wild-type BAF enhanced chromatin decondensation and nuclear growth; at higher added concentrations, wild-type BAF completely blocked chromatin decondensation and nuclear growth. Mutants fell into four classes, including one that defines a novel functional surface on the BAF dimer. Our results suggest that BAF, unregulated, potently compresses chromatin structure, and that BAF interactions with both DNA and LEM proteins are critical for membrane recruitment and chromatin decondensation during nuclear assembly.

Key Words: HIV; retroviral preintegration complex; nucleus; emerin; nuclear envelope

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

Related Article

BAF goes nuclear

Alan W. Dove
J. Cell Biol. 2002 158: 383. [Full Text] [PDF]

This article has been cited by other articles:

• Haraguchi, T., Kojidani, T., Koujin, T., Shimi, T., Osakada, H., Mori, C., Yamamoto, A., Hiraoka, Y. (2008). Live cell imaging and electron microscopy reveal dynamic processes of BAF-directed nuclear envelope assembly. J. Cell Sci. 121: 2540-2554 [Abstract] [Full Text]  
• Dahl, K. N., Ribeiro, A. J.S., Lammerding, J. (2008). Nuclear Shape, Mechanics, and Mechanotransduction. Circ. Res. 102: 1307-1318 [Abstract] [Full Text]  
• Resendes, K. K., Rasala, B. A., Forbes, D. J. (2008). Centrin 2 Localizes to the Vertebrate Nuclear Pore and Plays a Role in mRNA and Protein Export. Mol. Cell. Biol. 28: 1755-1769 [Abstract] [Full Text]  
• Brady, T. L., Fuerst, P. G., Dick, R. A., Schmidt, C., Voytas, D. F. (2008). Retrotransposon Target Site Selection by Imitation of a Cellular Protein. Mol. Cell. Biol. 28: 1230-1239 [Abstract] [Full Text]  
• Lancaster, O. M., Cullen, C. F., Ohkura, H. (2007). NHK-1 phosphorylates BAF to allow karyosome formation in the Drosophila oocyte nucleus. JCB 179: 817-824 [Abstract] [Full Text]  
• Chi, Y.-H., Haller, K., Peloponese, J.-M. Jr., Jeang, K.-T. (2007). Histone Acetyltransferase hALP and Nuclear Membrane Protein hsSUN1 Function in De-condensation of Mitotic Chromosomes. J. Biol. Chem. 282: 27447-27458 [Abstract] [Full Text]  
• Margalit, A., Neufeld, E., Feinstein, N., Wilson, K. L., Podbilewicz, B., Gruenbaum, Y. (2007). Barrier to autointegration factor blocks premature cell fusion and maintains adult muscle integrity in C. elegans. JCB 178: 661-673 [Abstract] [Full Text]  
• Haraguchi, T., Koujin, T., Osakada, H., Kojidani, T., Mori, C., Masuda, H., Hiraoka, Y. (2007). Nuclear localization of barrier-to-autointegration factor is correlated with progression of S phase in human cells. J. Cell Sci. 120: 1967-1977 [Abstract] [Full Text]  
• Morris, J. B., Hofemeister, H., O'Hare, P. (2007). Herpes Simplex Virus Infection Induces Phosphorylation and Delocalization of Emerin, a Key Inner Nuclear Membrane Protein. J. Virol. 81: 4429-4437 [Abstract] [Full Text]  
• Naetar, N., Hutter, S., Dorner, D., Dechat, T., Korbei, B., Gotzmann, J., Beug, H., Foisner, R. (2007). LAP2{alpha}-binding protein LINT-25 is a novel chromatin-associated protein involved in cell cycle exit. J. Cell Sci. 120: 737-747 [Abstract] [Full Text]  
• Shun, M.-C., Daigle, J. E., Vandegraaff, N., Engelman, A. (2007). Wild-Type Levels of Human Immunodeficiency Virus Type 1 Infectivity in the Absence of Cellular Emerin Protein. J. Virol. 81: 166-172 [Abstract] [Full Text]  
• Holaska, J. M., Rais-Bahrami, S., Wilson, K. L. (2006). Lmo7 is an emerin-binding protein that regulates the transcription of emerin and many other muscle-relevant genes. Hum Mol Genet 15: 3459-3472 [Abstract] [Full Text]  
• Nichols, R. J., Wiebe, M. S., Traktman, P. (2006). The Vaccinia-related Kinases Phosphorylate the N' Terminus of BAF, Regulating Its Interaction with DNA and Its Retention in the Nucleus. Mol. Biol. Cell 17: 2451-2464 [Abstract] [Full Text]  
• Bengtsson, L., Wilson, K. L. (2006). Barrier-to-Autointegration Factor Phosphorylation on Ser-4 Regulates Emerin Binding to Lamin A In Vitro and Emerin Localization In Vivo. Mol. Biol. Cell 17: 1154-1163 [Abstract] [Full Text]  
• de Oca, R. M., Lee, K. K., Wilson, K. L. (2005). Binding of Barrier to Autointegration Factor (BAF) to Histone H3 and Selected Linker Histones Including H1.1. J. Biol. Chem. 280: 42252-42262 [Abstract] [Full Text]  
• Brachner, A., Reipert, S., Foisner, R., Gotzmann, J. (2005). LEM2 is a novel MAN1-related inner nuclear membrane protein associated with A-type lamins. J. Cell Sci. 118: 5797-5810 [Abstract] [Full Text]  
• Hirano, Y., Segawa, M., Ouchi, F. S., Yamakawa, Y., Furukawa, K., Takeyasu, K., Horigome, T. (2005). Dissociation of Emerin from Barrier-to-autointegration Factor Is Regulated through Mitotic Phosphorylation of Emerin in a Xenopus Egg Cell-free System. J. Biol. Chem. 280: 39925-39933 [Abstract] [Full Text]  
• Roth, M. J., Forbes, A. J., Boyne, M. T. II, Kim, Y.-B., Robinson, D. E., Kelleher, N. L. (2005). Precise and Parallel Characterization of Coding Polymorphisms, Alternative Splicing, and Modifications in Human Proteins by Mass Spectrometry. Mol. Cell. Proteomics 4: 1002-1008 [Abstract] [Full Text]  
• Mansharamani, M., Wilson, K. L. (2005). Direct Binding of Nuclear Membrane Protein MAN1 to Emerin in Vitro and Two Modes of Binding to Barrier-to-Autointegration Factor. J. Biol. Chem. 280: 13863-13870 [Abstract] [Full Text]  
• Margalit, A., Segura-Totten, M., Gruenbaum, Y., Wilson, K. L. (2005). Barrier-to-autointegration factor is required to segregate and enclose chromosomes within the nuclear envelope and assemble the nuclear lamina. Proc. Natl. Acad. Sci. USA 102: 3290-3295 [Abstract] [Full Text]  
• Dechat, T., Gajewski, A., Korbei, B., Gerlich, D., Daigle, N., Haraguchi, T., Furukawa, K., Ellenberg, J., Foisner, R. (2004). LAP2{alpha} and BAF transiently localize to telomeres and specific regions on chromatin during nuclear assembly. J. Cell Sci. 117: 6117-6128 [Abstract] [Full Text]  
• Muchir, A., Worman, H. J. (2004). The Nuclear Envelope and Human Disease. Physiology 19: 309-314 [Abstract] [Full Text]  
• Zastrow, M. S., Vlcek, S., Wilson, K. L. (2004). Proteins that bind A-type lamins: integrating isolated clues. J. Cell Sci. 117: 979-987 [Abstract] [Full Text]  
• Mansharamani, M., Graham, D. R. M., Monie, D., Lee, K. K., Hildreth, J. E. K., Siliciano, R. F., Wilson, K. L. (2003). Barrier-to-Autointegration Factor BAF Binds p55 Gag and Matrix and Is a Host Component of Human Immunodeficiency Virus Type 1 Virions. J. Virol. 77: 13084-13092 [Abstract] [Full Text]  
• de Lahondes, R., Ribes, V., Arcangioli, B. (2003). Fission Yeast Sap1 Protein Is Essential for Chromosome Stability. Eukaryot Cell 2: 910-921 [Abstract] [Full Text]  
• Furukawa, K., Sugiyama, S., Osouda, S., Goto, H., Inagaki, M., Horigome, T., Omata, S., McConnell, M., Fisher, P. A., Nishida, Y. (2003). Barrier-to-autointegration factor plays crucial roles in cell cycle progression and nuclear organization in Drosophila. J. Cell Sci. 116: 3811-3823 [Abstract] [Full Text]  
• Schoft, V. K., Beauvais, A. J., Lang, C., Gajewski, A., Prufert, K., Winkler, C., Akimenko, M.-A., Paulin-Levasseur, M., Krohne, G. (2003). The lamina-associated polypeptide 2 (LAP2) isoforms {beta}, {gamma} and {omega} of zebrafish: developmental expression and behavior during the cell cycle. J. Cell Sci. 116: 2505-2517 [Abstract] [Full Text]  
• Osada, S.-I., Ohmori, S.-y., Taira, M. (2003). XMAN1, an inner nuclear membrane protein, antagonizes BMP signaling by interacting with Smad1 in Xenopus embryos. Development 130: 1783-1794 [Abstract] [Full Text]  
• Lin, C.-W., Engelman, A. (2003). The Barrier-to-Autointegration Factor Is a Component of Functional Human Immunodeficiency Virus Type 1 Preintegration Complexes. J. Virol. 77: 5030-5036 [Abstract] [Full Text]  
• Liu, J., Lee, K. K., Segura-Totten, M., Neufeld, E., Wilson, K. L., Gruenbaum, Y. (2003). MAN1 and emerin have overlapping function(s) essential for chromosome segregation and cell division in Caenorhabditis elegans. Proc. Natl. Acad. Sci. USA 100: 4598-4603 [Abstract] [Full Text]  
• Holaska, J. M., Lee, K. K., Kowalski, A. K., Wilson, K. L. (2003). Transcriptional Repressor Germ Cell-less (GCL) and Barrier to Autointegration Factor (BAF) Compete for Binding to Emerin in Vitro. J. Biol. Chem. 278: 6969-6975 [Abstract] [Full Text]  
• Martins, S., Eikvar, S., Furukawa, K., Collas, P. (2003). HA95 and LAP2{beta} mediate a novel chromatin-nuclear envelope interaction implicated in initiation of DNA replication. JCB 160: 177-188 [Abstract] [Full Text]  
• Wang, X., Xu, S., Rivolta, C., Li, L. Y., Peng, G.-H., Swain, P. K., Sung, C.-H., Swaroop, A., Berson, E. L., Dryja, T. P., Chen, S. (2002). Barrier to Autointegration Factor Interacts with the Cone-Rod Homeobox and Represses Its Transactivation Function. J. Biol. Chem. 277: 43288-43300 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents