Remodeling of organelle-bound actin is required for yeast vacuole fusion

doi:10.1083/jcb.200204089

Published online 12 August 2002. doi:10.1083/jcb.200204089

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© The Rockefeller University Press, 0021-9525/2002/8/669 $5.00
The Journal of Cell Biology, Volume 158, Number 4, August 19, 2002 669-679

Article

Remodeling of organelle-bound actin is required for yeast vacuole fusion

Gary Eitzen, Li Wang, Naomi Thorngren and William Wickner

Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03755

Address correspondence to William Wickner, Dept. of Biochemistry, Dartmouth Medical School, 7200 Vail Bldg., Hanover, NH 03755-3844. Tel.: (603) 650-1701. Fax: (603) 650-1353

Actin participates in several intracellular trafficking pathways.We now find that actin, bound to the surface of purified yeastvacuoles in the absence of cytosol or cytoskeleton, regulatesthe last compartment mixing stage of homotypic vacuole fusion.The Cdc42p GTPase is known to be required for vacuole fusion.We now show that proteins of the Cdc42p-regulated actin remodelingcascade (Cdc42p $->$ Cla4p $->$ Las17p/Vrp1p $->$ Arp2/3 complex $->$ actin)are enriched on isolated vacuoles. Vacuole fusion is dramaticallyaltered by perturbation of the vacuole-bound actin, either bymutation of the ACT1 gene, addition of specific actin ligandssuch as latrunculin B or jasplakinolide, antibody to the actinregulatory proteins Las17p (yeast Wiskott-Aldrich syndrome protein)or Arp2/3, or deletion of actin regulatory genes. On dockedvacuoles, actin is enriched at the "vertex ring" membrane microdomainwhere fusion occurs and is required for the terminal steps leadingto membrane fusion. This role for actin may extend to othertrafficking systems.

Key Words: yeast vacuoles; membrane fusion; actin; latrunculin B; jasplakinolide

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