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Published 3 September 2002. doi:10.1083/jcb.200202054
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© The Rockefeller University Press, 0021-9525/2002/9/901 $5.00
The Journal of Cell Biology, Volume 158, Number 5, September 2, 2002 901-914

Article

The CeCDC-14 phosphatase is required for cytokinesis in the Caenorhabditis elegans embryo



Ulrike Gruneberg1, Michael Glotzer2, Anton Gartner1 and Erich A. Nigg1

1 Department of Cell Biology, Max-Planck-Institute for Biochemistry, D-82152 Martinsried, Germany
2 Research Institute of Molecular Pathology, A-1030 Vienna, Austria

Address correspondence to Erich A. Nigg, Dept. of Cell Biology, Max-Planck-Institute of Biochemistry, Am Klopferspitz 18a, D-82152 Martinsried, Germany. Tel.: 49-89-8578-3100. Fax: 49-89-8578-3102. E-mail: nigg{at}biochem.mpg.de

In all eukaryotic organisms, the physical separation of two nascent cells must be coordinated with chromosome segregation and mitotic exit. In Saccharomyces cerevisiae and Schizosaccharomyces pombe this coordination depends on a number of genes that cooperate in intricate regulatory pathways termed mitotic exit network and septum initiation network, respectively. Here we have explored the function of potentially homologous genes in a metazoan organism, Caenorhabditis elegans, using RNA-mediated interference. Of all the genes tested, only depletion of CeCDC-14, the C. elegans homologue of the budding yeast dual-specificity phosphatase Cdc14p (Clp1/Flp1p in fission yeast), caused embryonic lethality. We show that CeCDC-14 is required for cytokinesis but may be dispensable for progression of the early embryonic cell cycles. In response to depletion of CeCDC-14, embryos fail to establish a central spindle, and several proteins normally found at this structure are mislocalized. CeCDC-14 itself localizes to the central spindle in anaphase and to the midbody in telophase. It colocalizes with the mitotic kinesin ZEN-4, and the two proteins depend on each other for correct localization. These findings identify the CDC14 phosphatase as an important regulator of central spindle formation and cytokinesis in a metazoan organism.

Key Words: CDC14; cytokinesis; central spindle; ZEN-4; AIR-2


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