Involvement of TRPC in the abnormal calcium influx observed in dystrophic (mdx) mouse skeletal muscle fibers

doi:10.1083/jcb.200203091

Published 16 September 2002. doi:10.1083/jcb.200203091

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© The Rockefeller University Press, 0021-9525/2002/9/1089 $5.00
The Journal of Cell Biology, Volume 158, Number 6, September 16, 2002 1089-1096

Article

Involvement of TRPC in the abnormal calcium influx observed in dystrophic (mdx) mouse skeletal muscle fibers

Clarisse Vandebrouck, Dominique Martin, Monique Colson-Van Schoor, Huguette Debaix and Philippe Gailly

Département de Physiologie, Université Catholique de Louvain (UCL 5540), 1200 Brussels, Belgium

Address correspondence to P. Gailly, Département de Physiologie, Université Catholique de Louvain (UCL 5540), Av. Hippocrate 55, 1200 Brussels, Belgium. Tel.: 32-2-764-55-42. Fax: 32-2-764-55-80. E-mail: gailly{at}fymu.ucl.ac.be

Duchenne muscular dystrophy results from the lack of dystrophin,a cytoskeletal protein associated with the inner surface membrane,in skeletal muscle. The absence of dystrophin induces an abnormalincrease of sarcolemmal calcium influx through cationic channelsin adult skeletal muscle fibers from dystrophic (mdx) mice.We observed that the activity of these channels was increasedafter depletion of the stores of calcium with thapsigargin orcaffeine. By analogy with the situation observed in nonexcitablecells, we therefore hypothesized that these store-operated channelscould belong to the transient receptor potential channel (TRPC)family. We measured the expression of TRPC isoforms in normaland mdx adult skeletal muscles fibers, and among the seven knownisoforms, five were detected (TRPC1, 2, 3, 4, and 6) by RT-PCR.Western blot analysis and immunocytochemistry of normal andmdx muscle fibers demonstrated the localization of TRPC1, 4,and 6 proteins at the plasma membrane. Therefore, an antisensestrategy was used to repress these TRPC isoforms. In parallelwith the repression of the TRPCs, we observed that the occurrenceof calcium leak channels was decreased to one tenth of its controlvalue (patch-clamp technique), showing the involvement of TRPCin the abnormal calcium influx observed in dystrophic fibers.

Key Words: calcium channels; dystrophy; sarcoplasmic reticulum; capacitative entry; TRP channel

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