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© The Rockefeller University Press, 0021-9525/2002/10/45 $5.00
The Journal of Cell Biology, Volume 159, Number 1, 45-53

Both midzone and astral microtubules are involved in the delivery of cytokinesis signals

Maki Murata-Hori and Yu-li Wang

University of Massachusetts Medical School, Worcester, MA 01605

Address correspondence to Yu-li Wang, University of Massachusetts Medical School, 377 Plantation St., Room 327, Worcester, MA 01605. Tel.: (508) 856-8781. Fax: (508) 856-8774. E-mail: yuli.wang{at}umassmed.edu

To address the mechanism that coordinates cytokinesis with mitosis, we have studied the dynamics of aurora B, a chromosomal passenger protein involved in signaling cytokinesis. Photobleaching analyses indicated dynamic exchange of aurora B between a centromeric and a cytoplasmic pool before anaphase onset, and stable associations with microtubules after anaphase onset. Bleaching near centromeres upon anaphase onset affected the subsequent appearance of fluorescence along midzone microtubules, but not that near the lateral equatorial cortex, suggesting that there were centromeric-dependent and -independent pathways that transported aurora B to the equator. The former delivered centromeric aurora B along midzone microtubules, whereas the latter delivered cytoplasmic aurora B along astral microtubules. We suggest that cultured cells use midzone microtubules as the primary signaling pathway for cytokinesis, whereas embryos, with their stockpile of cytoplasmic proteins and large sizes, rely primarily on astral microtubules.

Key Words: kinase; cell division; GFP; micromanipulation; photobleaching

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