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Published 25 November 2002. doi:10.1083/jcb.200205089
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© The Rockefeller University Press, 0021-9525/2002/11/557 $5.00
The Journal of Cell Biology, Volume 159, Number 4, 557-562

Report

K-loop insertion restores microtubule depolymerizing activity of a "neckless" MCAK mutant



Yulia Ovechkina, Michael Wagenbach and Linda Wordeman

Department of Physiology and Biophysics, University of Washington School of Medicine, Seattle, WA 98195

Address correspondence to Linda Wordeman, Dept. of Physiology and Biophysics, University of Washington, 1959 NE Pacific St., Box 357290, Seattle, WA 98195-7290. Tel.: (206) 543-4135. Fax: (206) 685-0619. E-mail: worde{at}u.washington.edu

Unlike most kinesins, mitotic centromere–associated kinesin (MCAK) does not translocate along the surface of microtubules (MTs), but instead depolymerizes them. Among the motile kinesins, refinements that are unique for specific cellular functions, such as directionality and processivity, are under the control of a "neck" domain adjacent to the ATP-hydrolyzing motor domain. Despite its apparent lack of motility, MCAK also contains a neck domain. We found that deletions and alanine substitutions of highly conserved positively charged residues in the MCAK neck domain significantly reduced MT depolymerization activity. Furthermore, substitution of MCAK's neck domain with either the positively charged KIF1A K-loop or poly-lysine rescues the loss of MT-depolymerizing activity observed in the neckless MCAK mutant. We propose that the neck, analogously to the K-loop, interacts electrostatically with the tubulin COOH terminus to permit diffusional translocation of MCAK along the surface of MTs. This weak-binding interaction may also play an important role in processivity of MCAK-induced MT depolymerization.

Key Words: Kin I; XKCM1; kinesins; processivity; diffusional motility


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