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Published 25 November 2002. doi:10.1083/jcb.200205084
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© The Rockefeller University Press, 0021-9525/2002/11/601 $5.00
The Journal of Cell Biology, Volume 159, Number 4, 601-611

Article

Iqg1p links spatial and secretion landmarks to polarity and cytokinesis



Mahasin A. Osman1, James B. Konopka2 and Richard A. Cerione1

1 Department of Molecular Medicine, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853
2 Department of Molecular Genetics and Microbiology, State University of New York, Stony Brook, NY 11794

Address correspondence to Mahasin A. Osman, Department of Molecular Medicine, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853-6401. Tel.: (607) 253-3883. Fax: (607) 253-3659. E-mail: mo28{at}cornell.edu

Cytokinesis requires the polarization of the actin cytoskeleton, the secretion machinery, and the correct positioning of the division axis. Budding yeast cells commit to their cytokinesis plane by choosing a bud site and polarizing their growth. Iqg1p (Cyk1p) was previously implicated in cytokinesis (Epp and Chant, 1997; Lippincott and Li, 1998; Osman and Cerione, 1998), as well as in the establishment of polarity and protein trafficking (Osman and Cerione, 1998). To better understand how Iqg1p influences these processes, we performed a two-hybrid screen and identified the spatial landmark Bud4p as a binding partner. Iqg1p can be coimmunoprecipitated with Bud4p, and Bud4p requires Iqg1p for its proper localization. Iqg1p also appears to specify axial bud-site selection and mediates the proper localization of the septin, Cdc12p, as well as binds and helps localize the secretion landmark, Sec3p. The double mutants iqg1{Delta}sec3{Delta} and bud4{Delta}sec3{Delta} display defects in polarity, budding pattern and cytokinesis, and electron microscopic studies reveal that these cells have aberrant septal deposition. Taken together, these findings suggest that Iqg1p recruits landmark proteins to form a targeting patch that coordinates axial budding with cytokinesis.

Key Words: Iqg1; Bud4; Sec3; polarity; cytokinesis


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