Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

This Article Full Text Full Text (PDF, 522K) PPT slides of all figures Alert me when this article is cited Citation Map Services Email this article Similar articles in this journal Similar articles in PubMed Alert me to new content in the JCB Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Nakai, Y. Articles by Kamiguchi, H. Search for Related Content PubMed PubMed Citation Articles by Nakai, Y. Articles by Kamiguchi, H. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB CHOLESTEROL Related Collections Related Article Social Bookmarking                      What's this?

© The Rockefeller University Press, 0021-9525/2002/12/1097 $5.00
The Journal of Cell Biology, Volume 159, Number 6, 1097-1108

Migration of nerve growth cones requires detergent-resistant membranes in a spatially defined and substrate-dependent manner

Developmental Brain Science Group, RIKEN Brain Science Institute (BSI), Wako, Saitama, 351-0198, Japan

Address correspondence to Hiroyuki Kamiguchi, Developmental Brain Science Group, RIKEN Brain Science Institute (BSI), 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan. Tel.: 81-48-467-6137. Fax: 81-48-467-9744. E-mail: kamiguchi{at}brain.riken.go.jp

Motility of nerve growth cones (GCs) is regulated by region-specific activities of cell adhesion molecules (CAMs). CAM activities could be modified by their localization to detergent-resistant membranes (DRMs), specialized microdomains enriched in signaling molecules. This paper deals with a question of whether DRMs are involved in GC migration stimulated by three CAMs; L1, N-cadherin (Ncad), and ß1 integrin. We demonstrate that L1 and Ncad are present in DRMs, whereas ß1 integrin is exclusively detected in non-DRMs of neurons and that localization of L1 and Ncad to DRMs is developmentally regulated. GC migration mediated by L1 and Ncad but not by ß1 integrin is inhibited after DRM disruption by micro-scale chromophore-assisted laser inactivation (micro-CALI) of GM1 gangliosides or by pharmacological treatments that deplete cellular cholesterol or sphingolipids, essential components for DRMs. Characteristic morphology of GCs induced by L1 and Ncad is also affected by micro-CALI–mediated DRM disruption. Micro-CALI within the peripheral domain of GCs, or even within smaller areas such as the filopodia and the lamellipodia, is sufficient to impair their migration. However, micro-CALI within the central domain does not affect GC migration. These results demonstrate the region-specific involvement of DRMs in CAM-dependent GC behavior.

Key Words: lipid raft; micro-CALI; neuron; L1; N-cadherin

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

Related Article

Shining a light on growth cones

Alan W. Dove
J. Cell Biol. 2002 159: 910. [Full Text] [PDF]

This article has been cited by other articles:

• Ichikawa, N., Iwabuchi, K., Kurihara, H., Ishii, K., Kobayashi, T., Sasaki, T., Hattori, N., Mizuno, Y., Hozumi, K., Yamada, Y., Arikawa-Hirasawa, E. (2009). Binding of laminin-1 to monosialoganglioside GM1 in lipid rafts is crucial for neurite outgrowth. J. Cell Sci. 122: 289-299 [Abstract] [Full Text]  
• Yuyama, K., Sekino-Suzuki, N., Sanai, Y., Kasahara, K. (2007). Translocation of Activated Heterotrimeric G Protein G{alpha}o to Ganglioside-enriched Detergent-resistant Membrane Rafts in Developing Cerebellum. J. Biol. Chem. 282: 26392-26400 [Abstract] [Full Text]  
• Wang, W., Mullikin-Kilpatrick, D., Crandall, J. E., Gronostajski, R. M., Litwack, E. D., Kilpatrick, D. L. (2007). Nuclear Factor I Coordinates Multiple Phases of Cerebellar Granule Cell Development via Regulation of Cell Adhesion Molecules. J. Neurosci. 27: 6115-6127 [Abstract] [Full Text]  
• Huang, J., Sakai, R., Furuichi, T. (2006). The Docking Protein Cas Links Tyrosine Phosphorylation Signaling to Elongation of Cerebellar Granule Cell Axons. Mol. Biol. Cell 17: 3187-3196 [Abstract] [Full Text]  
• Thoumine, O., Lambert, M., Mege, R.-M., Choquet, D. (2006). Regulation of N-Cadherin Dynamics at Neuronal Contacts by Ligand Binding and Cytoskeletal Coupling. Mol. Biol. Cell 17: 862-875 [Abstract] [Full Text]  
• Ooashi, N., Futatsugi, A., Yoshihara, F., Mikoshiba, K., Kamiguchi, H. (2005). Cell adhesion molecules regulate Ca2+-mediated steering of growth cones via cyclic AMP and ryanodine receptor type 3. JCB 170: 1159-1167 [Abstract] [Full Text]  
• Santuccione, A., Sytnyk, V., Leshchyns'ka, I., Schachner, M. (2005). Prion protein recruits its neuronal receptor NCAM to lipid rafts to activate p59fyn and to enhance neurite outgrowth. JCB 169: 341-354 [Abstract] [Full Text]  
• Falk, J., Thoumine, O., Dequidt, C., Choquet, D., Faivre-Sarrailh, C. (2004). NrCAM Coupling to the Cytoskeleton Depends on Multiple Protein Domains and Partitioning into Lipid Rafts. Mol. Biol. Cell 15: 4695-4709 [Abstract] [Full Text]  
• Nishimura, K., Yoshihara, F., Tojima, T., Ooashi, N., Yoon, W., Mikoshiba, K., Bennett, V., Kamiguchi, H. (2003). L1-dependent neuritogenesis involves ankyrinB that mediates L1-CAM coupling with retrograde actin flow. JCB 163: 1077-1088 [Abstract] [Full Text]  
• Buhusi, M., Midkiff, B. R., Gates, A. M., Richter, M., Schachner, M., Maness, P. F. (2003). Close Homolog of L1 Is an Enhancer of Integrin-mediated Cell Migration. J. Biol. Chem. 278: 25024-25031 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents