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Published online 27 January 2003. doi:10.1083/jcb.200207160
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© The Rockefeller University Press, 0021-9525/2003/2/313 $5.00
The Journal of Cell Biology, Volume 160, Number 3, 313-319

Report

Binding site for p120/{delta}-catenin is not required for Drosophila E-cadherin function in vivo



Anne Pacquelet, Li Lin and Pernille Rørth

European Molecular Biology Laboratory, 69117 Heidelberg, Germany

Address correspondence to Pernille Rørth, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany. Tel.: 49 6221 387109. Fax: 49 6221 387166. E-mail: rorth{at}EMBL-Heidelberg.de

Homophilic cell adhesion mediated by classical cadherins is important for many developmental processes. Proteins that interact with the cytoplasmic domain of cadherin, in particular the catenins, are thought to regulate the strength and possibly the dynamics of adhesion. ß-catenin links cadherin to the actin cytoskeleton via {alpha}-catenin. The role of p120/{delta}-catenin proteins in regulating cadherin function is less clear. Both ß-catenin and p120/{delta}-catenin are conserved in Drosophila. Here, we address the importance of cadherin–catenin interactions in vivo, using mutant variants of Drosophila epithelial cadherin (DE-cadherin) that are selectively defective in p120ctn (DE-cadherin-AAA) or ß-catenin–armadillo (DE-cadherin-{Delta}ß) interactions. We have analyzed the ability of these proteins to substitute for endogenous DE-cadherin activity in multiple cadherin-dependent processes during Drosophila development and oogenesis; epithelial integrity, follicle cell sorting, oocyte positioning, as well as the dynamic adhesion required for border cell migration. As expected, DE-cadherin-{Delta}ß did not substitute for DE-cadherin in these processes, although it retained some residual activity. Surprisingly, DE-cadherin-AAA was able to substitute for the wild-type protein in all contexts with no detectable perturbations. Thus, interaction with p120/{delta}-catenin does not appear to be required for DE-cadherin function in vivo.

Key Words: cell adhesion; oogenesis; migration; morphogenesis; adherens junction


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