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Published 3 February 2003. doi:10.1083/jcb.200209018
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© The Rockefeller University Press, 0021-9525/2003/2/329 $5.00
The Journal of Cell Biology, Volume 160, Number 3, 329-339

Article

The budding yeast Ipl1/Aurora protein kinase regulates mitotic spindle disassembly



Stéphanie Buvelot1,2, Sean Y. Tatsutani1, Danielle Vermaak1 and Sue Biggins1

1 Fred Hutchinson Cancer Research Center, Seattle, WA 98109
2 University of Fribourg, 1700 Fribourg, Switzerland

Address correspondence to Fred Hutchinson Cancer Research Center, P.O. Box 19024, 1100 Fairview Ave., N. (A2-168), Seattle, WA 98109-1024. Tel.: (206) 667-1351. Fax: (206) 667-6526. E-mail: sbiggins{at}fhcrc.org

Ipl1p is the budding yeast member of the Aurora family of protein kinases, critical regulators of genomic stability that are required for chromosome segregation, the spindle checkpoint, and cytokinesis. Using time-lapse microscopy, we found that Ipl1p also has a function in mitotic spindle disassembly that is separable from its previously identified roles. Ipl1–GFP localizes to kinetochores from G1 to metaphase, transfers to the spindle after metaphase, and accumulates at the spindle midzone late in anaphase. Ipl1p kinase activity increases at anaphase, and ipl1 mutants can stabilize fragile spindles. As the spindle disassembles, Ipl1p follows the plus ends of the depolymerizing spindle microtubules. Many Ipl1p substrates colocalize with Ipl1p to the spindle midzone, identifying additional proteins that may regulate spindle disassembly. We propose that Ipl1p regulates both the kinetochore and interpolar microtubule plus ends to regulate its various mitotic functions.

Key Words: Ipl1/Aurora protein kinase; spindle; mitosis; microtubule; budding yeast


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