Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

This Article Full Text Full Text (PDF, 672K) PPT slides of all figures Alert me when this article is cited Citation Map Services Email this article Similar articles in this journal Similar articles in PubMed Alert me to new content in the JCB Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Cuvier, O. Articles by Hirano, T. Search for Related Content PubMed PubMed Citation Articles by Cuvier, O. Articles by Hirano, T. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB TENIPOSIDE Social Bookmarking                            What's this?

© The Rockefeller University Press, 0021-9525/2003/3/645 $5.00
The Journal of Cell Biology, Volume 160, Number 5, 645-655

A role of topoisomerase II in linking DNA replication to chromosome condensation

Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724

Address correspondence to Tatsuya Hirano, Cold Spring Harbor Laboratory, 1 Bungtown Rd., Cold Spring Harbor, NY 11724. Tel.: (516) 367-8370. Fax: (516) 367-8815. E-mail: hirano{at}cshl.org

The condensin complex and topoisomerase II (topo II) have different biochemical activities in vitro, and both are required for mitotic chromosome condensation. We have used Xenopus egg extracts to investigate the functional interplay between condensin and topo II in chromosome condensation. When unreplicated chromatin is directly converted into chromosomes with single chromatids, the two proteins must function together, although they are independently targeted to chromosomes. In contrast, the requirement for topo II is temporarily separable from that of condensin when chromosome assembly is induced after DNA replication. This experimental setting allows us to find that, in the absence of condensin, topo II becomes enriched in an axial structure within uncondensed chromatin. Subsequent addition of condensin converts this structure into mitotic chromosomes in an ATP hydrolysis–dependent manner. Strikingly, preventing DNA replication by the addition of geminin or aphidicolin disturbs the formation of topo II–containing axes and alters the binding property of topo II with chromatin. Our results suggest that topo II plays an important role in an early stage of chromosome condensation, and that this function of topo II is tightly coupled with prior DNA replication.

Key Words: Xenopus egg extracts; condensin; decatenation; compaction; SMC

CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

• Roca, J. (2009). Topoisomerase II: a fitted mechanism for the chromatin landscape. Nucleic Acids Res 37: 721-730 [Abstract] [Full Text]  
• Cuvier, O., Stanojcic, S., Lemaitre, J.-M., Mechali, M. (2008). A topoisomerase II-dependent mechanism for resetting replicons at the S-M-phase transition. Genes Dev. 22: 860-865 [Abstract] [Full Text]  
• Kirik, V., Schrader, A., Uhrig, J. F., Hulskamp, M. (2007). MIDGET Unravels Functions of the Arabidopsis Topoisomerase VI Complex in DNA Endoreduplication, Chromatin Condensation, and Transcriptional Silencing. Plant Cell 19: 3100-3110 [Abstract] [Full Text]  
• Linka, R. M., Porter, A. C.G., Volkov, A., Mielke, C., Boege, F., Christensen, M. O. (2007). C-Terminal regions of topoisomerase II{alpha} and II{beta} determine isoform-specific functioning of the enzymes in vivo. Nucleic Acids Res 35: 3810-3822 [Abstract] [Full Text]  
• Lee, C.-P., Chen, J.-Y., Wang, J.-T., Kimura, K., Takemoto, A., Lu, C.-C., Chen, M.-R. (2007). Epstein-Barr Virus BGLF4 Kinase Induces Premature Chromosome Condensation through Activation of Condensin and Topoisomerase II. J. Virol. 81: 5166-5180 [Abstract] [Full Text]  
• Takemoto, A., Murayama, A., Katano, M., Urano, T., Furukawa, K., Yokoyama, S., Yanagisawa, J., Hanaoka, F., Kimura, K. (2007). Analysis of the role of Aurora B on the chromosomal targeting of condensin I. Nucleic Acids Res 35: 2403-2412 [Abstract] [Full Text]  
• Zhang, A., Lyu, Y. L., Lin, C.-P., Zhou, N., Azarova, A. M., Wood, L. M., Liu, L. F. (2006). A Protease Pathway for the Repair of Topoisomerase II-DNA Covalent Complexes. J. Biol. Chem. 281: 35997-36003 [Abstract] [Full Text]  
• Lyu, Y. L., Lin, C.-P., Azarova, A. M., Cai, L., Wang, J. C., Liu, L. F. (2006). Role of Topoisomerase II{beta} in the Expression of Developmentally Regulated Genes. Mol. Cell. Biol. 26: 7929-7941 [Abstract] [Full Text]  
• Cobbe, N., Savvidou, E., Heck, M. M. S. (2006). Diverse Mitotic and Interphase Functions of Condensins in Drosophila. Genetics 172: 991-1008 [Abstract] [Full Text]  
• Marchion, D. C., Bicaku, E., Turner, J. G., Daud, A. I., Sullivan, D. M., Munster, P. N. (2005). Synergistic Interaction between Histone Deacetylase and Topoisomerase II Inhibitors Is Mediated through Topoisomerase II{beta}. Clin. Cancer Res. 11: 8467-8475 [Abstract] [Full Text]  
• Savvidou, E., Cobbe, N., Steffensen, S., Cotterill, S., Heck, M. M. S. (2005). Drosophila CAP-D2 is required for condensin complex stability and resolution of sister chromatids. J. Cell Sci. 118: 2529-2543 [Abstract] [Full Text]  
• Dej, K. J., Ahn, C., Orr-Weaver, T. L. (2004). Mutations in the Drosophila Condensin Subunit dCAP-G: Defining the Role of Condensin for Chromosome Condensation in Mitosis and Gene Expression in Interphase. Genetics 168: 895-906 [Abstract] [Full Text]  
• Harvey, S. H., Sheedy, D. M., Cuddihy, A. R., O'Connell, M. J. (2004). Coordination of DNA Damage Responses via the Smc5/Smc6 Complex. Mol. Cell. Biol. 24: 662-674 [Abstract] [Full Text]  
• Coelho, P. A., Queiroz-Machado, J., Sunkel, C. E. (2003). Condensin-dependent localisation of topoisomerase II to an axial chromosomal structure is required for sister chromatid resolution during mitosis. J. Cell Sci. 116: 4763-4776 [Abstract] [Full Text]  
• Siddiqui, N. U., Stronghill, P. E., Dengler, R. E., Hasenkampf, C. A., Riggs, C. D. (2003). Mutations in Arabidopsis condensin genes disrupt embryogenesis, meristem organization and segregation of homologous chromosomes during meiosis. Development 130: 3283-3295 [Abstract] [Full Text]  
• Watrin, E., Cubizolles, F., Osborne, H. B., Le Guellec, K., Legagneux, V. (2003). Expression and Functional Dynamics of the XCAP-D2 Condensin Subunit in Xenopus laevis Oocytes. J. Biol. Chem. 278: 25708-25715 [Abstract] [Full Text]  
• Yang, X., Makaroff, C. A., Ma, H. (2003). The Arabidopsis MALE MEIOCYTE DEATH1 Gene Encodes a PHD-Finger Protein That Is Required for Male Meiosis. Plant Cell 15: 1281-1295 [Abstract] [Full Text]  

Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents