The RasGAP-associated endoribonuclease G3BP assembles stress granules

doi:10.1083/jcb.200212128

Published 17 March 2003. doi:10.1083/jcb.200212128

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© The Rockefeller University Press, 0021-9525/2003/3/823 $5.00
The Journal of Cell Biology, Volume 160, Number 6, 823-831

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The RasGAP-associated endoribonuclease G3BP assembles stress granules

Hélène Tourrière, Karim Chebli, Latifa Zekri, Brice Courselaud, Jean Marie Blanchard, Edouard Bertrand and Jamal Tazi

Institut de Génétique Moléculaire de Montpellier, UMR 5535 du Centre National de la Recherche Scientifique (CNRS), Université Montpellier II, 34293 Montpellier, Cedex 5, France

Address correspondence to Jamal Tazi, Institut de Génétique Moléculaire de Montpellier, UMR 5535 du CNRS, IFR 122, 1919 Route de Mende, 34293 Montpellier, Cedex 5, France. Tel.: 33 (0) 4-67-61-36-85. Fax: 33 (0) 4-67-04-02-31. E-mail: tazi{at}igm.cnrs-mop.fr

Stress granules (SGs) are formed in the cytoplasm in responseto various toxic agents, and are believed to play a criticalrole in the regulation of mRNA metabolism during stress. InSGs, mRNAs are stored in an abortive translation initiationcomplex that can be routed to either translation initiationor degradation. Here, we show that G3BP, a phosphorylation-dependentendoribonuclease that interacts with RasGAP, is recruited toSGs in cells exposed to arsenite. G3BP may thus determine thefate of mRNAs during cellular stress. Remarkably, SG assemblycan be either dominantly induced by G3BP overexpression, oron the contrary, inhibited by expressing a central domain ofG3BP. This region binds RasGAP and contains serine 149, whosedephosphorylation is induced by arsenite treatment. Critically,a phosphomimetic mutant (S149E) fails to oligomerize and toassemble SGs, whereas a nonphosphorylatable G3BP mutant (S149A)does both. These results suggest that G3BP is an effector ofSG assembly, and that Ras signaling contributes to this processby regulating G3BP dephosphorylation.

Key Words: mRNA stability; protein phosphorylation; mammalian endoribonucleases; Ras signaling; RNA-binding protein

H. Tourrière and K. Chebli contributed equally to thispaper.

^* Abbreviations used in this paper: RRM, RNA recognition motif;Ser 149, serine 149; SG, stress granule.

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