JCB logo
R&D Systems: New Poster Available
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published 17 March 2003. doi:10.1083/jcb.200211116
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 500K)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mythreye, K.
Right arrow Articles by Bloom, K. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mythreye, K.
Right arrow Articles by Bloom, K. S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Facebook   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

© The Rockefeller University Press, 0021-9525/2003/3/833 $5.00
The Journal of Cell Biology, Volume 160, Number 6, 833-843

Article

Differential kinetochore protein requirements for establishment versus propagation of centromere activity in Saccharomyces cerevisiae



Karthikeyan Mythreye and Kerry S. Bloom

Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599

Address correspondence to Kerry S. Bloom, 622 Fordham Hall, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599. Tel.: (919) 962-1182. Fax: (919) 962-8461. E-mail: kbloom{at}email.unc.edu

Dicentric chromosomes undergo a breakage–fusion–bridge cycle as a consequence of having two centromeres on the same chromatid attach to opposite spindle poles in mitosis. Suppression of dicentric chromosome breakage reflects loss of kinetochore function at the kinetochore–microtubule or the kinetochore–DNA interface. Using a conditionally functional dicentric chromosome in vivo, we demonstrate that kinetochore mutants exhibit quantitative differences in their degree of chromosome breakage. Mutations in chl4/mcm17/ctf17 segregate dicentric chromosomes through successive cell divisions without breakage, indicating that only one of the two centromeres is functional. Centromere DNA introduced into the cell is unable to promote kinetochore assembly in the absence of CHL4. In contrast, established centromeres retain their segregation capacity for greater than 25 generations after depletion of Chl4p. The persistent mitotic stability of established centromeres reveals the presence of an epigenetic component in kinetochore segregation. Furthermore, this study identifies Chl4p in the initiation and specification of a heritable chromatin state.

Key Words: yeast; centromere; kinetochore; chromatin; epigenetic

* Abbreviation used in this paper: ChIP, chromatin immunoprecipitation.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Facebook Facebook   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents