An anillin homologue, Mid2p, acts during fission yeast cytokinesis to organize the septin ring and promote cell separation

doi:10.1083/jcb.200211126

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Published 31 March 2003. doi:10.1083/jcb.200211126

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© The Rockefeller University Press, 0021-9525/2003/3/1093 $5.00
The Journal of Cell Biology, Volume 160, Number 7, 1093-1103

Article

An anillin homologue, Mid2p, acts during fission yeast cytokinesis to organize the septin ring and promote cell separation

Joseph J. Tasto², Jennifer L. Morrell¹^,2 and Kathleen L. Gould¹^,2

¹ Howard Hughes Medical Institute, Vanderbilt University School of Medicine, Nashville, TN 37232
² Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN 37232

Address correspondence to Kathleen L. Gould, Vanderbilt University School of Medicine, Department of Cell and Developmental Biology, B2309 MCN, 1161 21st Ave. South, Nashville, TN 37232. Tel.: (615) 343-9502. Fax: (615) 343-0723. E-mail: kathy.gould{at}vanderbilt.edu

Anillin is a conserved protein required for cell division (Field,C.M., and B.M. Alberts. 1995. J. Cell Biol. 131:165–178;Oegema, K., M.S. Savoian, T.J. Mitchison, and C.M. Field. 2000.J. Cell Biol. 150:539–552). One fission yeast homologueof anillin, Mid1p, is necessary for the proper placement ofthe division site within the cell (Chang, F., A. Woollard, andP. Nurse. 1996. J. Cell Sci. 109(Pt 1):131–142; Sohrmann,M., C. Fankhauser, C. Brodbeck, and V. Simanis. 1996. GenesDev. 10:2707–2719). Here, we identify and characterizea second fission yeast anillin homologue, Mid2p, which is notorthologous with Mid1p. Mid2p localizes as a single ring inthe middle of the cell after anaphase in a septin- and actin-dependentmanner and splits into two rings during septation. Mid2p colocalizeswith septins, and mid2 ${Delta}$ cells display disorganized, diffuse septinrings and a cell separation defect similar to septin deletionstrains. mid2 gene expression and protein levels fluctuate duringthe cell cycle in a sep1- and Skp1/Cdc53/F-box (SCF)–dependentmanner, respectively, implying that Mid2p activity must be carefullyregulated. Overproduction of Mid2p depolarizes cell growth andaffects the organization of both the septin and actin cytoskeletons.In the presence of a nondegradable Mid2p fragment, the septinring is stabilized and cell cycle progression is delayed. Theseresults suggest that Mid2p influences septin ring organizationat the site of cell division and its turnover might normallybe required to permit septin ring disassembly.

Key Words: S. pombe; cytokinesis; cell cycle; septin; mid2

The online version of this article includes supplemental material.

^* Abbreviations used in this paper: APC, anaphase-promoting complex;LatA, latrunculin A; PEST, Pro/Glu/Ser/Thr; PH, pleckstrin homology;SCF, Skp1/Cdc53/F-box.

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