JCB logo
PeproTech: Cell Culture Supplements
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
Published 14 April 2003. doi:10.1083/jcb.200208099
This Article
Right arrow Full Text
Right arrow Full Text (PDF, 316K)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Citation Map
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JCB
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Colanzi, A.
Right arrow Articles by Malhotra, V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Colanzi, A.
Right arrow Articles by Malhotra, V.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

© The Rockefeller University Press, 0021-9525/2003/4/27 $5.00
The Journal of Cell Biology, Volume 161, Number 1, 27-32

Report

RAF1-activated MEK1 is found on the Golgi apparatus in late prophase and is required for Golgi complex fragmentation in mitosis



Antonino Colanzi, Christine Sutterlin and Vivek Malhotra

Cell and Developmental Biology, University of California, San Diego, La Jolla, CA 92093

Address correspondence to Vivek Malhotra, Cell and Developmental Biology Department, B0347, University of California, San Diego, Pacific Hall, 2nd Floor, Rm. 2222A, La Jolla, CA 92093-0347. Tel.: (858) 534-8910. Fax: (858) 534-0555. E-mail: malhotra{at}biomail.ucsd.edu

Amitotically activated mitogen-activated protein kinase 1 (MEK1) fragments the pericentriolar Golgi stacks in mammalian cells. We show that activated MEK1 is found on the Golgi apparatus in late prophase. The fragmented and dispersed Golgi membranes in prometaphase and later stages of mitosis do not contain activated MEK1. MEK1-dependent Golgi complex fragmentation is through activation by RAF1 and not MEK1 kinase 1. We propose that a RAF1-dependent activation of MEK1 and its presence on the Golgi apparatus in late prophase is required for Golgi complex fragmentation.

Key Words: mitogen-activated protein kinase kinase 1; cell cycle; organelle inheritance; RKIP; phosphorylation

* Abbreviations used in this paper: ERK, extracellular signal-regulated kinase; ManII, mannosidase II; MEK1, mitogen-activated protein kinase 1; MEKK1, MEK kinase 1; NRK, normal rat kidney; ppMEK, phospho-MEK; RKIP, RAF1 kinase inhibitory protein.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents