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© The Rockefeller University Press, 0021-9525/2003/4/67 $5.00
The Journal of Cell Biology, Volume 161, Number 1, 67-77

Arrest of mammalian fibroblasts in G1 in response to actin inhibition is dependent on retinoblastoma pocket proteins but not on p53

Institut de Biologie Structurale Jean Pierre Ebel (Commissariat à l'Energie Atomique–Centre National de la Recherche Scientifique–Université Joseph Fourier), 38027 Grenoble cedex 1, France

Address correspondence to Robert L. Margolis, Institut de Biologie Structurale, 41 rue Jules Horowitz, 38027 Grenoble cedex 1, France. Tel.: (33) 4-38-78-9616. Fax: (33) 4-38-78-5494. E-mail: margolis{at}ibs.fr

p53 and the retinoblastoma (RB) pocket proteins are central to the control of progression through the G1 phase of the cell cycle. The RB pocket protein family is downstream of p53 and controls S-phase entry. Disruption of actin assembly arrests nontransformed mammalian fibroblasts in G1. We show that this arrest requires intact RB pocket protein function, but surprisingly does not require p53. Thus, mammalian fibroblasts with normal pocket protein function reversibly arrest in G1 on exposure to actin inhibitors regardless of their p53 status. By contrast, pocket protein triple knockout mouse embryo fibroblasts and T antigen–transformed rat embryo fibroblasts lacking both p53 and RB pocket protein function do not arrest in G1. Fibroblasts are very sensitive to actin inhibition in G1 and arrest at drug concentrations that do not affect cell adhesion or cell cleavage. Interestingly, G1 arrest is accompanied by inhibition of surface ruffling and by induction of NF2/merlin. The combination of failure of G1 control and of tetraploid checkpoint control can cause RB pocket protein–suppressed cells to rapidly become aneuploid and die after exposure to actin inhibitors, whereas pocket protein–competent cells are spared. Our results thus establish that RB pocket proteins can be uniquely targeted for tumor chemotherapy.

Key Words: aneuploidy; tetraploidy; cytoskeleton; dihydrocytochalasin B; cell cycle

^* Abbreviations used in this paper: ARF, alternative reading frame; CKI, Cdk inhibitor; DCB, dihydrocytochalasin B; ERK, extracellular signal–regulated kinase; HU, hydroxyurea; MEF, mouse embryo fibroblast; RB, retinoblastoma; REF, rat embryo fibroblast.

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