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Published online 21 April 2003. doi:10.1083/jcb.200207117
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© The Rockefeller University Press, 0021-9525/2003/4/229 $5.00
The Journal of Cell Biology, Volume 161, Number 2, 229-236

Report

CSC-1

: a subunit of the aurora b kinase complex that binds to the survivin-like protein BIR-1 and the incenp-like protein ICP-1



Alper Romano1, Annika Guse1, Ivica Krascenicova1, Heinke Schnabel2, Ralf Schnabel2 and Michael Glotzer1

1 Research Institute of Molecular Pathology, A-1030 Vienna, Austria
2 Technical University Braunschweig, D-38106 Braunschweig, Germany

Address correspondence to Michael Glotzer, Research Institute of Molecular Pathology, Dr. Bohrgasse 7, Vienna A-1030, Austria. Tel.: 43-1-797-30-405. Fax: 43-1-798-7153. E-mail: mglotzer{at}nt.imp.univie.ac.at

The Aurora B kinase complex is a critical regulator of chromosome segregation and cytokinesis. In Caenorhabditis elegans, AIR-2 (Aurora B) function requires ICP-1 (Incenp) and BIR-1 (Survivin). In various systems, Aurora B binds to orthologues of these proteins. Through genetic analysis, we have identified a new subunit of the Aurora B kinase complex, CSC-1. C. elegans embryos depleted of CSC-1, AIR-2, ICP-1, or BIR-1 have identical phenotypes. CSC-1, BIR-1, and ICP-1 are interdependent for their localization, and all are required for AIR-2 localization. In vitro, CSC-1 binds directly to BIR-1. The CSC-1/BIR-1 complex, but not the individual subunits, associates with ICP-1. CSC-1 associates with ICP-1, BIR-1, and AIR-2 in vivo. ICP-1 dramatically stimulates AIR-2 kinase activity. This activity is not stimulated by CSC-1/BIR-1, suggesting that these two subunits function as targeting subunits for AIR-2 kinase.

Key Words: chromosome segregation; cytokinesis; central spindle; IAP; C. elegans

The online version of this article includes supplemental material.

H. Schnabel's present address is Max Planck Institut fur Neurobiologie, D-82152 Martinstried, Germany.

* Abbreviations used in this paper: ABI, Aurora B, BIR-1, and Incenp; BIR, baculovirus IAP repeat; CBD, chitin-binding domain; MBP, myelin basic protein; RNAi, RNA interference; SNP, single nucleotide polymorphism.


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